Preservation of rat skeletal muscle energy metabolism by illumination
Preservation of rat skeletal muscle energy metabolism by illumination
复制标题
DOI：
10.1016/s0024-3205(03)00176-0
复制
发表时间：
2003-04-25
期刊：
影响因子：
6.1
通讯作者：
Soussi, B
中科院分区：
文献类型：
--
作者：
Lindgård, A;Lundberg, J;Soussi, B
骨骼肌的活力是至关重要的取决于其高能量磷酸盐的组织水平。在这项研究中，我们研究了保存介质Perfadex和照明与单线态氧能量（SOE）的效果。单线态氧可以通过从激发的光敏剂的能量转移而光化学地产生。从单线态氧在弛豫到其三重态时发射的能量在634 nm处被捕获为光子，并且在此被称为SOE。将大鼠后肢股直肌在Perfadex、生理盐水、SOE照射的Perfadex或SOE照射的生理盐水中于22 ℃保存5小时。通过P-31 NMR分析肌肉提取物。使用双因素方差分析分析数据，并以平均值（μ mol/g干重）SEM给出。生理盐水组的ATP浓度（4.52）高于Perfadex组（2.82）（p = 0.006）。生理盐水组（1.25）和Perfadex组（0.82）之间的PCr无统计学显著差异。然而，与非照明组（2.73）相比，SOE照明组（4.61）中的ATP更高（p = 0.003）。SOE照射组的PCr（1.89）也高于非照射组（0.18）（P < 0.0001）。总之，在该实验模型中，Perfadex在5小时缺血期间不能保留骨骼肌中的高能磷酸盐。在634 nm处使用SOE的照明提高了保存潜力，对肌肉细胞的能量状态具有积极影响。(C)2003年爱思唯尔科学公司All rights reserved.
Skeletal muscle viability is crucially dependent on the tissue levels of its high energy phosphates. In this study we investigated the effect of the preservation medium Perfadex and illumination with Singlet Oxygen Energy (SOE). Singlet oxygen can be produced photochemically by energy transfer from an excited photosensitizer. The energy emitted from singlet oxygen upon relaxation to its triplet state is captured as photons at 634 nm and is here referred to as SOE. Rat hind limb rectus femoris muscles were preserved for five hours at 22 degreesC in Perfadex, saline, SOE illuminated Perfadex or SOE illuminated saline. Extracts of the muscles were analysed by P-31 NMR. Data were analysed using two-way analysis of variance and are given as mean values (mumol/g dry weight) SEM. The ATP concentration was higher (p = 0.006) in saline groups (4.52) compared with Perfadex groups (2.82). There was no statistically significant difference in PCr between the saline groups (1.25) and Perfadex groups (0.82). However, there were higher (p = 0.003) ATP in the SOE illuminated groups (4.61) compared with the non-illuminated groups (2.73). The PCr was also higher (P < 0.0001) in the SOE illuminated groups (1.89) compared with the non-illuminated groups (0.18). In conclusion, Perfadex in this experimental model was incapable of preserving the high energy phosphates in skeletal muscle during 5 hours of ischemia. Illumination with SOE at 634 nm improved the preservation potential, in terms of a positive effect on the energy status of the muscle cell. (C) 2003 Elsevier Science Inc. All rights reserved.