Local renal and vascular natriuretic peptide system in obstructive uropathic rats
Local renal and vascular natriuretic peptide system in obstructive uropathic rats
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DOI：
10.1007/s00240-002-0242-9
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发表时间：
2002-05
影响因子：
--
通讯作者：
S. Kim;Yingshun Li;Sunmi Kim;Y. Oh;Jongun Lee
中科院分区：
文献类型：
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作者：
S. Kim;Yingshun Li;Sunmi Kim;Y. Oh;Jongun Lee
本研究旨在探讨梗阻性尿路病患者肾脏和血管系统局部利钠肽系统的调节是否改变。雄性Sprague-Dawley大鼠通过结扎近端输尿管48 h造成双侧梗阻。以相同的方式处理对照大鼠，不同之处在于不进行结扎。通过逆转录-聚合酶链反应测定肾脏和胸主动脉中心房、脑和C型利钠肽（ANP、BNP、CNP）的各种亚型以及利钠肽受体-A、-B和-C（NPR-A、NPR-B、NPR-C）的不同亚型的mRNA表达。还检查了颗粒鸟苷酸环化酶的基础和刺激活性。在双侧输尿管梗阻后，ANP、BNP和CNP在主动脉和肾脏中的表达增加。与此相反，在肾脏和主动脉中NPR-A、NPR-B和NPR-C的表达均降低。因此，鸟苷酸环化酶活性显着降低，以响应利钠肽。ANP以剂量依赖性方式舒张苯肾上腺素预收缩的主动脉环，其程度显著降低。我们的研究结果表明，局部合成的利钠肽增加，在肾脏和血管阻塞性尿路病。
The present study was aimed at investigating whether the regulation of the local natriuretic peptide system is altered in the kidney and the vasculature in obstructive uropathy. Male Sprague-Dawley rats were bilaterally obstructed by ligation of the proximal ureters for 48 h. Control rats were treated in the same way, except that no ligature was made. The mRNA expression of the various isoforms of atrial, brain, and C-type natriuretic peptide (ANP, BNP, CNP) and different subtypes of natriuretic peptide receptor-A, -B, and -C (NPR-A, NPR-B, NPR-C) was determined in the kidney and the thoracic aorta by reverse transcription-polymerase chain reaction. The basal and stimulated activities of particulate guanylyl cyclase were also examined. Following the bilateral ureteral obstruction, the expression of ANP, BNP, and CNP was increased in the aorta as well as in the kidney. Contrary to this, the expression of NPR-A, NPR-B, and NPR-C was decreased both in the kidney and the aorta. Accordingly, the guanylyl cyclase activity was significantly decreased in response to natriuretic peptides. ANP relaxed phenylephrine-precontracted aortic rings in a dose-dependent manner, the degree of which was significantly diminished. Our results suggest that the local synthesis of natriuretic peptides is increased in the kidney and in the vasculature in obstructive uropathy.