The nucleotide sequence of the Pieris brassicae granulosis virus granulin gene.
The nucleotide sequence of the Pieris brassicae granulosis virus granulin gene.
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菜粉蝶颗粒病毒颗粒蛋白基因的核苷酸序列。
DOI：
10.1099/0022-1317-66-6-1263
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Beaudreau,GS
中科院分区：
文献类型：
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作者：
Chakerian,R;Rohrmann,GF;Nesson,MH;Leisy,DJ;Beaudreau,GS
将含有菜粉蝶颗粒病毒(GV)颗粒蛋白基因的两个重叠限制性片段克隆到质粒中。将包含编码区以及 5' 和 3' 侧翼区的区域亚克隆到 M13 中并测序。将核苷酸序列数据与来自粉纹夜蛾GV的颗粒蛋白基因和来自苜蓿银纹夜蛾核多角体病毒(AcMNPV)的多角体蛋白基因的数据进行比较。从这些 DNA 序列衍生的氨基酸序列表明，这两种 GV 蛋白彼此之间的相关性（77% 氨基酸同源性）比任一与 AcMNPV 的相关性（任一 GV 的氨基酸同源性约为 53%）更为密切。 N 末端区域显示这些蛋白质之间最大程度的变异。在两个 GV 之间以及 NPV 之间也发现了高度保守的氨基酸序列。其中某些保留区域在 GV 和 NPV 之间共享，而其他区域则不然。
Two overlapping restriction fragments containing thePieris brassicaegranulosis virus (GV) granulin gene were cloned into plasmids. The regions containing the coding region and the 5′ and 3′ flanking regions were subcloned into M13 and sequenced. The nucleotide sequence data were compared to those for the granulin gene from theTrichoplusia niGV and the polyhedrin gene from theAutographa californicanuclear polyhedrosis virus (AcMNPV). The amino acid sequences derived from these DNA sequences indicated that the two GV proteins are more closely related to each other (77% amino acid homology) than either is to the AcMNPV (about 53% amino acid homology for either GV). The N-terminal region shows the greatest degree of variation between these proteins. Highly conserved amino acid sequences were identified between the two GVs and were also found between NPVs. Certain of these conserved regions are shared between GVs and NPVs while others are not.