Prevent Effects of Lactobacillus Fermentum HY01 on Dextran Sulfate Sodium-Induced Colitis in Mice.
Prevent Effects of Lactobacillus Fermentum HY01 on Dextran Sulfate Sodium-Induced Colitis in Mice.
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作者：
Chen X;Zhao X;Wang H;Yang Z;Li J;Suo H
本研究旨在评价发酵乳杆菌HY01(LF-HY01)对葡聚糖硫酸钠诱导的结肠炎的预防作用。观察大鼠结肠重量与长度的比值、结肠病理变化及血清中促炎因子(干扰素-γ、白介素12、肿瘤坏死因子α和白介素6)的浓度。同时观察IκBα、NF-κB p65、iNOS和COX-2的蛋白表达水平，观察不同剂量发酵乳杆菌HY01(低剂量组109cfu/kg·bw，高剂量组1010cfu/kg·bw)对葡聚糖硫酸钠诱导的小鼠结肠炎的影响。结果表明，发酵乳杆菌HY01对葡聚糖硫酸钠诱导的结肠炎有明显的预防作用，同时高剂量组效果最好。能有效缓解结肠长度缩短，降低结肠重量与长度的比值，减轻水肿、炎性细胞浸润和结肠黏膜损伤，并在下调促炎因子(干扰素-γ、IL-12、肿瘤坏死因子α和IL-6)浓度方面发挥重要作用。最重要的是，发酵乳杆菌HY01对IκBα的降解、抑制NF-κB p65的磷酸化级联反应、以及降低iNOS和COX-2的蛋白水平都显示出很好的预防作用。
The aim of this study is to assess the preventive effects of Lactobacillus fermentum HY01 (LF-HY01) to dextran sulfate sodium induced-colitis. We observed the ratio of colon weight to its length, colon pathological changes, and the concentrations of pro-inflammatory factors (IFN-γ, IL-12, TNFα, and IL-6) in serum. We also took account of the protein levels of IκBα, NF-κB p65, iNOS, and COX-2, and we measured the best effects of different doses of Lactobacillus fermentum HY01 (low dose group was 109 CFU/kg·bw, high dose group was 1010 CFU/kg·bw) on dextran sulfate sodium-induced colitis mice. The results were remarkable, suggesting that Lactobacillus fermentum HY01 had significant preventive effects in dextran sulfate sodium induced-colitis; simultaneously, the high dose group showed the best results among other groups. It can effectively alleviate the shortened colon length, reduce the ratio of colon weight to its length, reduce edema, inflammatory cells infiltration, and colon mucosa injury, and play an important role in the down-regulation of concentrations of pro-inflammatory factors (IFN-γ, IL-12, TNFα, and IL-6). Above all, Lactobacillus fermentum HY01 shows promising prevention for IκBα degradation, inhibition of NF-κB p65 phosphorylation cascades, and decreases the protein levels of iNOS and COX-2 as well.