Primary structure of twenty three neutral and monosialylated oligosaccharides O-glycosidically linked to the human secretory immunoglobulin A hinge region determined by a combination of permethylation analysis and 400-MHz 1H-NMR spectroscopy.
Primary structure of twenty three neutral and monosialylated oligosaccharides O-glycosidically linked to the human secretory immunoglobulin A hinge region determined by a combination of permethylation analysis and 400-MHz 1H-NMR spectroscopy.
复制标题
通过全甲基化分析和 400-MHz 1H-NMR 光谱相结合测定，二十三种中性单唾液酸化寡糖的一级结构与人分泌性免疫球蛋白 A 铰链区 O-糖苷连接。
DOI：
10.1111/j.1432-1033.1989.tb14853.x
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
G. Spik
中科院分区：
文献类型：
--
作者：
A. Pierce;J. Decottignies;J. Wieruszeski;G. Strecker;J. Montreuil;G. Spik
唾液低聚糖和唾液低聚糖醛醇来源于人乳分泌免疫球蛋白A铰链区，先后采用氨基键合硅胶柱和十八烷基键合硅胶柱进行高效液相色谱纯化。通过糖分析、甲基化质谱和400-MHz 1H-NMR谱的结合，完全解析了它们的一级结构。在本报告中，描述了23种低聚糖醛醇，它们都具有由支链三糖组成的二型核心：Gal(β 1-3)[GlcNAc(β 1-6)]GalNAc-ol。对于唾液化的化合物，仅在GlcNAc（β 1-6）分支上的链延长导致链延长，导致十二糖，而对于中性化合物，两个分支上的链延长导致非糖。所描述的低聚糖结构中有16种是原始的。此外，一些聚焦结构被发现支持Le(a)、Le(b)和X血型决定因子。
Sialooligosaccharide and asialooligosaccharide alditols, derived from the human milk secretory immunoglobulin A hinge region, have been purified by HPLC using, successively, an amino-bonded silica column and an octadecyl-bonded silica column. Their primary structures were completely resolved by applying a combination of sugar analysis, methylation mass spectrometry and 400-MHz 1H-NMR spectroscopy. In the present report, twenty three oligosaccharide alditols are described and all possess a type two core consisting of the branched trisaccharide: Gal(beta 1-3)[GlcNAc(beta 1-6)]GalNAc-ol. The elongation of this core arises by chain lengthening only on the GlcNAc(beta 1-6) branch for the sialylated compounds, leading to a dodecasaccharide, and on both branches for the neutral compounds leading to a nonasaccharide. Sixteen of the described oligosaccharide structures are original. Moreover, some of the fucosylated structures were found to support Le(a), Le(b) and X blood group determinants.
人 IgD 的 O-糖苷连接寡糖的结构。
DOI：
--
发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Mellis,SJ;Baenziger,JU
通讯作者：
Baenziger,JU