Mini chamber system for long-term maintenance and observation of cultured cells
Mini chamber system for long-term maintenance and observation of cultured cells
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DOI：
10.2144/05382rr03
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发表时间：
2005-02-01
期刊：
影响因子：
2.7
通讯作者：
Chow, NH
中科院分区：
文献类型：
--
作者：
Ho, CL;Mou, TY;Chow, NH
我们构建了一个能够在显微镜上长时间保持细胞培养的迷你小室系统。它是一种改良的密闭式系统，具有中等灌流和二氧化碳循环。密闭的CO循环和室内充足的空气使其有别于其他密闭系统。使用不同的细胞系，该系统被证明能够支持长期的延时记录。在229小时的延时记录后，A2058细胞(一种黑色素瘤细胞系)变得过度融合，但仍在增殖。许多CAD细胞(一种小鼠神经元样细胞系)在28天的记录后仍然移动其胞体并保持其突起样突起。可以监测擦伤T24(一种膀胱癌细胞系)单层的整个愈合过程。这种改进的封闭系统应该会在发育生物学、细胞生物学和癌症生物学中得到许多应用，在这些领域需要长期的延时记录或当细胞的健康很重要时。
We constructed a mini chamber system that was able to maintain cell culture on a microscope for long periods. It is a modified closed system with medium perfusion and CO2 circulation. The closed CO circulation and ample air inside the chamber distinguish it from other closed systems. Using different cell lines, the system was shown to be able to support long-term, time-lapse recording. After 229 hours of time-lapse recording, A2058 cells (a melanoma cell line) became overconfluent but still multiplied. Many CAD cells (a murine neuron-like cell line) still moved their cell bodies and kept their neurite-like processes after 28 days of recording. The entire healing process of a scratch-wounded T24 (a bladder cancer cell line) monolayer can be monitored. Such a modified closed system should find many applications in developmental biology, cell biology, and cancer biology where long-term, time-lapse recording is required or when the health of cells is important.