CNS microvascular pericytes express macrophage-like function, cell surface integrin alpha M, and macrophage marker ED-2
CNS microvascular pericytes express macrophage-like function, cell surface integrin alpha M, and macrophage marker ED-2
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DOI：
10.1006/mvre.1996.0049
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发表时间：
1996-09-01
影响因子：
3.1
通讯作者：
DoreDuffy, P
中科院分区：
文献类型：
--
作者：
Balabanov, R;Washington, R;DoreDuffy, P
中枢神经系统周细胞是血脑屏障（BBB）的组成部分，但其功能还不清楚。我们质疑原代培养的中枢神经系统周细胞是否具有免疫潜力。原代培养的周细胞暴露于荧光染料结合的聚苯乙烯珠和抗体包被的酵母聚糖时表现出吞噬活性。最大的吞噬活性发生3小时。周细胞被发现表达巨噬细胞标志物艾德-2和整合素亚基CD 11 b（α M）在文化中，以及在分离的微血管。周细胞不表达巨噬细胞标志物艾德-1。我们证实了培养的中枢神经系统周细胞表达α-平滑肌肌动蛋白的异质性。总之，周细胞表达巨噬细胞表面抗原，并有能力执行至少一些免疫功能。因此，CNS周细胞可能在BBB的神经免疫网络中发挥作用。(C)出版社：Academic Press，Inc.
CNS pericytes are an integral part of the blood-brain barrier (BBB), but their function is not well understood. We questioned whether primary cultured CNS pericytes have immune potential. Primary cultured pericytes exhibit phagocytic activity when exposed to fluorochrome-conjugated polystyrene beads and antibody-coated zymosan. Maximum phagocytic activity occurred by 3 hr. Pericytes were found to express the macrophage markers ED-2 and the integrin subunit CD11b (alpha M) in culture as well as on isolated microvessels. Pericytes did not express the macrophage marker ED-1. We confirm the heterogeneity of cultured CNS pericytes with regard to expression of alpha-smooth muscle actin. In conclusion, pericytes express macrophage surface antigens and have the ability to perform at least some immune function. CNS pericytes may therefore have a role in neuroimmune networks at the BBB. (C) 1996 Academic Press, Inc.