Association of intrastrain phase variation in quantity of capsular polysaccharide and teichoic acid with the virulence of Streptococcus pneumoniae
Association of intrastrain phase variation in quantity of capsular polysaccharide and teichoic acid with the virulence of Streptococcus pneumoniae
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DOI：
10.1086/514205
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发表时间：
1998-02-01
影响因子：
6.4
通讯作者：
Weiser, JN
中科院分区：
文献类型：
--
作者：
Kim, JO;Weiser, JN
肺炎球菌在不透明和透明菌落形式之间经历自发相变化。在腹腔内接种小鼠后的全身感染动物模型中，对于所检查的3种菌株中的每一种，不透明表型比透明表型的毒性显著更强。使用夹心ELISA，与透明表型相比，不透明表型与1.2- 5.6倍的荚膜多糖量相关，一项类似的技术比较了总磷壁酸的量，结果表明透明表型具有2.1至3.8倍的免疫可检测磷壁酸。通过比较[H-3]胆碱掺入磷壁酸中证实了这种差异。细胞分级分离显示，掺入胆碱量的变化是由于细胞壁相关磷壁酸的差异。结果表明，肺炎球菌阶段之间的毒力形式与更多的荚膜多糖和更少的磷壁酸和无毒力的形式与更少的荚膜多糖和更多的磷壁酸。
The pneumococcus undergoes spontaneous phase variation between an opaque and a transparent colony form. In an animal model of systemic infection following intraperitoneal inoculation of mice, the opaque phenotype was significantly more virulent than the transparent for each of 3 strains examined, The opaque phenotype was associated with 1.2- to 5.6-fold greater amounts of capsular polysaccharide compared with the transparent using a sandwich ELISA, A similar technique comparing the amount of total teichoic acid showed that the transparent phenotype had 2.1- to 3.8-fold more immunodetectable teichoic acid. This difference was confirmed by comparing the incorporation of [H-3]choline into teichoic acid, Cell fractionation revealed that variation in quantity of incorporated choline was due to differences in cell wall-associated teichoic acid. Results suggest that the pneumococcus phase varies between a virulent form with more capsular polysaccharide and less teichoic acid and an avirulent form with less capsular polysaccharide and more teichoic acid.