EXPERIMENTAL HUMAN INFECTIONS WITH GIARDIA-LAMBLIA
EXPERIMENTAL HUMAN INFECTIONS WITH GIARDIA-LAMBLIA
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DOI：
10.1093/infdis/156.6.974
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发表时间：
1987-12-01
影响因子：
6.4
通讯作者：
LEVINE, MM
中科院分区：
文献类型：
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作者：
NASH, TE;HERRINGTON, DA;LEVINE, MM
15名健康志愿者肠道接种蓝氏贾第鞭毛虫GS/M株和IsR株滋养体。将50,000个滋养体(GS/M或IsR)接种给两组5名志愿者的ECH。所有志愿者接种GS/M疫苗，而接种ISR的志愿者均未被感染。感染GS/M的五名志愿者中有三人患病，其中两人有腹泻和贾第鞭毛虫病的典型症状。在第二项研究中，三名先前感染过GS/M并接受治疗的患者在第一次接种后12周再次接受挑战，还有五名新的对照志愿者。后一组的所有人都被感染了，两人出现了松弛的大便；两名再次接受挑战的志愿者再次感染，但没有症状，第三人被回顾发现在挑战时被感染。在感染的志愿者中，血清对贾第虫的IgM、IgG和IgA抗体应答以及肠液对贾第虫的IgA抗体应答的发生率分别为10%、70%、60%和50%。这些研究满足了科赫的S假设，并证明了在人类贾第虫感染的致病性中菌株的变异。
Fifteen healthy volunteers were inoculated enterally with trophozoites of two distinct human isolates of Giardia lamblia, GS/M and Isr. Ech of two groups of five volunteers were inoculated with 50,000 (GS/M or Isr) trophozoites. All of the volunteers inoculated with GS/M and none of the volunteers inoculated with Isr became infected. Three of five volunteers infected with GS/M became ill, including two who had diarrhea and typical symptoms of giardiasis. In the second study, three patients who were previously infected with GS/M and treated were rechallenged 12 weeks after the first inoculation, together with five new control volunteers. All of the latter group became infected, and two developed loose stools; two rechallenged volunteers became reinfected but were asymptomatic, and a third was retrospectively found to be infected at the time of challenge. Serum IgM, IgG, and IgA antibody responses to Giardia and intestinal fluid IgA antibody responses to Giardia occurred in 10%, 70%, 60%, and 50%, respectively, of infected volunteers. These studies fulfill Koch''s postulates and demonstrate strain variation in the pathogenicity of Giardia infections in humans.