Optical control of neuronal activities with photoswitchable nanovesicles
Optical control of neuronal activities with photoswitchable nanovesicles
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DOI：
10.1007/s12274-022-4853-x
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发表时间：
2022-06
期刊：
影响因子：
9.9
通讯作者：
H. Xiong;Kevin A Alberto;Jonghae Youn;Jaume J. Taura;Johannes Morstein;Xiuying Li;Yang Wang;
中科院分区：
文献类型：
--
作者：
H. Xiong;Kevin A Alberto;Jonghae Youn;Jaume J. Taura;Johannes Morstein;Xiuying Li;Yang Wang;
神经活性分子对神经元活动的精确调节对于理解大脑回路和行为至关重要。然而，缺乏用于高度可控的分子释放的工具。在这里，我们开发了一种光开关纳米囊泡与偶氮苯含磷脂酰胆碱（偶氮PC），创造了“偶氮体”，用于神经调节。365 nm光照射引发偶氮PC的反式-顺式异构化，导致脂质双层结构紊乱，厚度减少，货物释放减少。用455 nm光照射诱导反向异构化并关闭释放。实时荧光成像显示在几秒钟内（< 3秒）可控和可重复的货物释放。重要的是，我们证明，SKF-81297，多巴胺D1受体激动剂，可以反复释放的偶氮体激活培养的初级纹状体神经元。偶氮体在分子释放的精确光学控制方面显示出良好的前景，可以成为分子神经科学研究的一个有价值的工具。
Precise modulation of neuronal activity by neuroactive molecules is essential for understanding brain circuits and behavior. However, tools for highly controllable molecular release are lacking. Here, we developed a photoswitchable nanovesicle with azobenzene-containing phosphatidylcholine (azo-PC), coined ‘azosome’, for neuromodulation. Irradiation with 365 nm light triggers thetrans-to-cisisomerization of azo-PC, resulting in a disordered lipid bilayer with decreased thickness and cargo release. Irradiation with 455 nm light induces reverse isomerization and switches the release off. Real-time fluorescence imaging shows controllable and repeatable cargo release within seconds (< 3 s). Importantly, we demonstrate that SKF-81297, a dopamine D1-receptor agonist, can be repeatedly released from the azosome to activate cultures of primary striatal neurons. Azosome shows promise for precise optical control over the molecular release and can be a valuable tool for molecular neuroscience studies.