Deciphering the Nature of Enzymatic Modifications of Bacterial Cell Walls
Deciphering the Nature of Enzymatic Modifications of Bacterial Cell Walls
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DOI：
10.1002/cbic.201700293
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发表时间：
2017-09-05
期刊：
影响因子：
3.2
通讯作者：
Mobashery, Shahriar
中科院分区：
文献类型：
--
作者：
Lee, Mijoon;Hesek, Dusan;Mobashery, Shahriar
细菌细胞壁的主要成分是肽聚糖，其交联形式是一种相当复杂的聚合物，包裹着整个细菌。功能性细胞壁对于生物体的生存是必不可少的。在每一代细菌中，有几十种酶动态地组装和分解肽聚糖。了解这些转变的性质是这些事件的关键知识。八糖肽聚糖的制备和研究与七个重组细胞壁活性酶（SltB1，MltB，RlpA，变溶菌素，AmpDh2，AmpDh3，PBP5）。使用高灵敏度的质谱方法，我们描述了这些酶与肽聚糖催化的反应的广度，并阐明了这些酶对细胞壁改变的性质。这些酶在其催化的反应中对底物肽聚糖表现出广泛不同的偏好。
The major constituent of bacterial cell walls is peptidoglycan, which, in its crosslinked form, is a polymer of considerable complexity that encases the entire bacterium. A functional cell wall is indispensable for survival of the organism. There are several dozen enzymes that assemble and disassemble the peptidoglycan dynamically within each bacterial generation. Understanding of the nature of these transformations is critical knowledge for these events. Octasaccharide peptidoglycans were prepared and studied with seven recombinant cell-wall-active enzymes (SltB1, MltB, RlpA, mutanolysin, AmpDh2, AmpDh3, and PBP5). With the use of highly sensitive mass spectrometry methods, we described the breadth of reactions that these enzymes catalyzed with peptidoglycan and shed light on the nature of the cell wall alteration performed by these enzymes. The enzymes exhibit broadly distinct preferences for their substrate peptidoglycans in the reactions that they catalyze.