PULSED-FIELD GEL-ELECTROPHORESIS APPLIED FOR COMPARING LISTERIA-MONOCYTOGENES STRAINS INVOLVED IN OUTBREAKS
PULSED-FIELD GEL-ELECTROPHORESIS APPLIED FOR COMPARING LISTERIA-MONOCYTOGENES STRAINS INVOLVED IN OUTBREAKS
复制标题
DOI：
10.1139/m93-058
复制
发表时间：
1993-04-01
影响因子：
2.8
通讯作者：
ROCOURT, J
中科院分区：
文献类型：
--
作者：
BUCHRIESER, C;BROSCH, R;ROCOURT, J
最近人类李斯特菌病的食源性暴发以及大量散发性病例主要是由单核细胞增多性李斯特菌4b血清型菌株引起的。因此，我们用脉冲场凝胶电泳法对APAI、SMAI或NOTI产生的大片段染色体DNA限制性内切酶片段进行了分析，对近期6次重大和8次较小规模的李斯特菌病暴发期间分离的75株单核细胞增多性李斯特菌进行了分析。这些菌株可分为20个不同的基因组变种。在瑞士(1983-1987年)、美国(加利福尼亚州，1985年)和丹麦(1985-1987年)主要疫情期间分离的14株病毒中，有13株显示出难以区分的DNA限制模式。相比之下，在加拿大(新斯科舍省，1981)、美国(马萨诸塞州，1983)、法国(Anjou，1975-1976)、新西兰(1969)和奥地利(1986)以及法国(1987、1988，)暴发的毒株都具有特定的DNA限制性图谱组合。77%的测试菌株可以归入前面描述的Apai组A(Brosch等人。1991)，显示了非常密切的基因组亲缘关系。由于本研究中49%的流行菌株属于噬菌体2389/2425/3274/2671/47/108/340或2389/47/108/340，因此还对分离自不同来源的另外56株噬菌体进行了分型，以确定是否可以指出同一噬菌体的流行菌株和随机选择的菌株之间的DNA限制性内切酶图谱的差异。DNA图谱的变异在随机选择的菌株中比在流行菌株中出现得更频繁。
Recent food-borne outbreaks of human listeriosis as well as numerous sporadic cases have been mainly caused by Listeria monocytogenes serovar 4b strains. Thus, it was of interest to find out whether a certain clone or a certain few clones were responsible for these cases and especially for outbreaks., We used pulsed-field gel electrophoresis of large chromosomal DNA restriction fragments generated by ApaI, SmaI, or NotI to analyse 75 L. monocytogenes strains isolated during six major and eight smaller recent listeriosis outbreaks. These strains could be divided into 20 different genomic varieties. Thirteen of 14 strains isolated during major epidemics in Switzerland (1983-1987), the United States (California, 1985) and Denmark (1985-1987) demonstrated indistinguishable DNA restriction patterns. In contrast, strains responsible for the outbreaks in Canada (Nova Scotia, 1981), the United States (Massachusetts, 1983), France (Anjou, 1975-1976), New Zealand (1969), and Austria (1986) and some smaller outbreaks in France (1987, 1988, 1989) were each characterized by particular combinations of DNA restriction patterns. Seventy-seven percent of the tested strains could be classified into the previously described ApaI group A (Brosch et al. 1991), demonstrating a very close genomic relatedness. Because 49% of the epidemic strains selected for this study belonged to phagovar 2389/2425/3274/2671/47/108/340 or 2389/47/108/340, fifty-six additional strains of these phagovars, isolated from various origins, were also typed to determine whether differences in DNA restriction profiles between epidemic and randomly selected strains of the same phagovars could be pointed out. Variations in DNA patterns appeared more frequently within randomly selected strains than within epidemic strains.