Differential expression of innate and adaptive immune genes in the survivors of three gibel carp gynogenetic clones after herpesvirus challenge
Differential expression of innate and adaptive immune genes in the survivors of three gibel carp gynogenetic clones after herpesvirus challenge
复制标题
疱疹病毒攻击后三个银鲤雌核发育克隆的幸存者中先天性和适应性免疫基因的差异表达
DOI：
10.1186/s12864-019-5777-z
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发表时间：
2019-05-28
期刊：
影响因子：
4.4
通讯作者：
Gui, Jian-Fang
中科院分区：
文献类型：
--
作者：
Lu, Wei-Jia;Gao, Fan-Xiang;Gui, Jian-Fang
【摘要】：背景伴随着银鲫的快速生长和高密度养殖,自2012年以来,银鲫受到金鲫疱疹病毒(CaHV)的严重威胁。在前期研究中,银鲫3个雌核发育无性系(A+、F和H)的患病个体均表现出明显的CaHV抵抗和免疫反应。然而，对于CaHV攻击后幸存者中基因表达的变化，特别是易感克隆和抗性克隆之间先天性和适应性免疫系统的差异知之甚少。结果我们首先通过评估5个CaHV基因的丰度，证实了3个银鲫鱼克隆在CaHV攻击后的幸存者中CaHV携带状态。病毒载量测定表明，抗性克隆H不仅具有较强的抗性，而且对CaHV具有较高的耐受性。然后，从幸存者的头肾转录组谱中筛选出2818、4047和3323个差异表达的unigenes（DEU），与克隆A+、F和H的对照进行比较。GO和KEGG分析表明，耐药克隆H和F可能维持持续的免疫反应，而易感克隆A+对循环系统有长期影响，这与CaHV引起的出血的主要症状一致。在各个克隆中特异性上调DEU的前30条富集通路中，克隆H、F和A+中分别有26、7和15条通路与感染、疾病或免疫相关通路相关。此外，克隆F中的20条通路属于“代谢”或“生物发生”，克隆A+中富集了7条涉及“循环系统”的通路。值得注意的是，我们揭示了三个克隆的幸存者中 IFN 系统基因和免疫球蛋白 (Ig) 基因的差异表达变化。最后，肌球蛋白和Igs被确定为共表达模块，分别与CaHV病毒载量呈正相关或负相关。结论我们的结果揭示了三个具有不同CaHV抗性的银鲫雌核发育无性系的幸存者中免疫和循环系统中共同且独特的基因表达变化。本研究代表了硬骨鱼先天性和适应性免疫反应差异的范例，将有利于银鲫的抗病育种。
BackgroundAccompanied with rapid growth and high density aquaculture, gibel carp has been seriously threatened byCarassius auratusherpesvirus (CaHV) since 2012. In previous study, distinctCaHV resistances and immune responses were revealed in the diseased individuals of three gibel carp gynogenetic clones (A+, F and H). However, little is known about the gene expression changes in the survivors afterCaHV challenge, particularly their differences of innate and adaptive immune system between susceptible clone and resistant clone.ResultsWe firstly confirmed theCaHV carrier state in the survivors of three gibel carp clones afterCaHV challenge by evaluating the abundances of fiveCaHV genes. The assay of viral loads indicated the resistant clone H possessed not only stronger resistance but also higher tolerance toCaHV. Then, 2818, 4047 and 3323 differentially expressed unigenes (DEUs) were screened from the head-kidney transcriptome profiles of survivors compared with controls from clone A+, F and H. GO and KEGG analysis suggested that a persistent immune response might sustain in resistant clone H and F, while susceptible clone A+had a long-term impact on the circulatory system which was consistent with the major symptoms of bleeding caused byCaHV. Among the top 30 enriched pathways of specifically up-regulated DEUs in respective clones, 26, 7 and 15 pathways in clone H, F and A+were associated with infections, diseases, or immune-related pathways respectively. In addition, 20 pathways in clone F belonged to “metabolism” or “biogenesis”, and 7 pathways involved in “circulatory system” were enriched in clone A+. Significantly, we revealed the differential expression changes of IFN system genes and immunoglobulin (Ig) genes among the survivors of three clones. Finally, myosins and Igs were identified as co-expression modules which were positively or negatively correlated toCaHV viral loads respectively.ConclusionsOur results revealed the common and distinct gene expression changes in immune and circulatory system in the survivors of three gibel carp gynogenetic clones with differentCaHV resistances. The current study represents a paradigm of differential innate and adaptive immune reactions in teleost, and will be beneficial to the disease-resistance breeding of gibel carp.