Sodium dodecyl sulfate-capillary electrophoresis of proteins in a sieving matrix utilizing two-spectral channel laser-induced fluorescence detection
Sodium dodecyl sulfate-capillary electrophoresis of proteins in a sieving matrix utilizing two-spectral channel laser-induced fluorescence detection
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DOI：
10.1002/elps.1150191222
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发表时间：
1998-09-01
期刊：
影响因子：
2.9
通讯作者：
Dovichi, NJ
中科院分区：
文献类型：
--
作者：
Craig, DB;Polakowski, PM;Dovichi, NJ
我们报告了一种蛋白质标记，在聚合物筛分基质中通过毛细管电泳分离，并通过激光诱导荧光检测的方法。不同的染料用于标记标准和样品蛋白质。双光谱通道检测器可分辨样品和标准品的荧光。通过比较样品和标准品的迁移时间，可以精确测定分子量，而不考虑运行间迁移时间的变化。
We report a method for protein labeling, separation by capillary electrophoresis in a polymer sieving matrix, and detection by laser-induced fluorescence. Different dyes are used to label standard and sample proteins. A two-spectral channel detector resolves fluorescence from the sample and standards. Comparison of the migration time of the sample and standards permits the precise determination of molecular weight, irrespective of variations in run-to-run migration times.