Circulating human microRNAs are not linked to JC polyomavirus serology or urinary viral load in healthy subjects.
Circulating human microRNAs are not linked to JC polyomavirus serology or urinary viral load in healthy subjects.
复制标题
循环的人类microRNA与健康受试者中的JC多瘤病毒血清学或尿病毒负荷无关。
DOI：
10.1186/1743-422x-11-41
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发表时间：
2014-03-03
期刊：
影响因子：
4.8
通讯作者：
Stuyver LJ
中科院分区：
文献类型：
--
作者：
Lagatie O;Van Loy T;Tritsmans L;Stuyver LJ
JC多瘤病毒（JC polyomavirus，JCPyV）是一种广泛分布的人类多瘤病毒，通常潜伏在其宿主中。它可以在免疫调节条件下重新激活，并引起进行性多灶性白质脑病（PML）。循环microRNA（miRNAs）正在成为几种病理学的有前途的生物标志物。在这项研究中，我们研究了一方面在JCPyV血清阳性和JCPyV血清阴性之间或另一方面在JCPyV脱落者和JCPyV非脱落者之间是否存在差异表达的循环miRNA。在从血清阴性受试者、血清阳性脱落者和血清阳性非脱落者获得的一小组血浆样品中进行人miRNA表达谱分析。选择一组10种miRNA用于在更大的样品组中进行进一步分析。基于30个样本的血浆谱分析实验，选择了6个可能在血清阳性和血清阴性受试者之间差异表达的miRNA，并且选择了4个可能在脱落者和非脱落者之间差异表达的miRNA。随后，在一组独立的100个血浆样品中评估这10种选择的miRNA的表达。结果表明，没有一个差异表达。该研究不能鉴定在来自JCPyV血清阳性和JCPyV血清阴性受试者的血浆之间或在JCPyV散发者和JCPyV非散发者之间差异表达的循环人miRNA。
JC polyomavirus (JCPyV) is a widespread human polyomavirus that usually resides latently in its host. It can be reactivated under immunomodulating conditions and cause Progressive Multifocal Leukoencephalopathy (PML). Circulating microRNAs (miRNAs) are emerging as promising biomarkers for several pathologies. In this study, we have investigated whether circulating miRNAs exist that are differentially expressed between JCPyV seropositive and JCPyV seronegative on the one hand or between JCPyV shedders and JCPyV non-shedders on the other hand. Human miRNA expression profiling was performed in a small set of plasma samples obtained from seronegative subjects, seropositive shedders and seropositive non-shedders. A set of 10 miRNAs was selected for further analysis in a larger group of samples. Based on the plasma profiling experiment of 30 samples, 6 miRNAs were selected that were possibly differentially expressed between seropositive and seronegative subjects and 4 miRNAs were selected that were possibly differentially expressed between shedders and non-shedders. Subsequently, expression of these 10 selected miRNAs was assessed in an independent set of 100 plasma samples. Results indicated that none of them were differentially expressed. This study could not identify circulating human miRNAs that were differentially expressed between plasma from JCPyV seropositive and JCPyV seronegative subjects or between JCPyV shedders and JCPyV non-shedders.