Fish cell lines: Establishment of a line from ovaries of channel catfish
Fish cell lines: Establishment of a line from ovaries of channel catfish
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DOI：
10.1007/bf02618357
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发表时间：
1980-05
期刊：
影响因子：
--
通讯作者：
P. Bowser;J. Plumb
中科院分区：
文献类型：
--
作者：
P. Bowser;J. Plumb
从健康斑点叉尾鮰幼鱼的卵巢中建立了细胞系。这些细胞被命名为 CCO，在 3 年内已通过 130 次。细胞在 Eagle 的 MEM-10 中在 30°C 的温度下生长良好。通过细胞毒性染料排除测试确认细胞来源的种类。发现这些细胞没有细菌和真菌污染。染色体制备的研究表明核型仍处于不断变化的状态。 CCO 系复制了斑点叉尾鮰病毒，但对其他 12 种病毒具有抵抗力，其中 4 种来自鱼类，1 种来自鸟类，7 种来自哺乳动物。
A cell line was established from the ovaries of a healthy, juvenile channel catfish,Ictalurus punctatus. These cells, designated CCO, have been passed 130 times during 3 years. The cells grow well in Eagle's MEM-10 at a temperature of 30°C. Species of origin of the cells was confirmed by a cytotoxic dye exclusion test. The cells were found to be free of bacterial and fungal contamination. A study of chromosome preparations indicated that the karyotype is still in a state of flux. The CCO line replicated channel catfish virus but was refractory to 12 other viruses, 4 from fish, 1 from birds, and 7 from mammals.