Comprehensive analysis of microRNA expression patterns in renal biopsies of lupus nephritis patients
Comprehensive analysis of microRNA expression patterns in renal biopsies of lupus nephritis patients
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DOI：
10.1007/s00296-008-0758-6
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发表时间：
2009-05-01
影响因子：
4
通讯作者：
Huang, YuanShuai
中科院分区：
文献类型：
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作者：
Dai, Yong;Sui, Weiguo;Huang, YuanShuai
MicroRNAs(MiRNAs)是21-24个核苷酸的非编码RNA分子，以转录后方式调节靶基因的表达。有证据表明，miRNAs在胚胎发育、细胞分化和人类疾病的发病机制中发挥着重要作用。本研究对狼疮性肾炎(LN)患者和对照组肾活检组织的miRNA图谱进行了比较，以进一步了解LN的发病机制。肾活检取自5例LN II级患者和3例正常对照。MiRNA芯片分析鉴定出66个在LN中差异表达的miRNAs。芯片检测结果经QRT-PCR证实。提示miRNAs是LN潜在的诊断标志物和可能参与LN发病的因素。
MicroRNAs (miRNAs) are noncoding RNA molecules of 21-24 nt that regulate the expression of target genes in a post-transcriptional manner. Evidence indicates that miRNAs play essential roles in embryogenesis, cell differentiation, and pathogenesis of human diseases. This study describes a comparison between the miRNA profile of kidney biopsies from lupus nephritis (LN) patients and the controls, to develop further understanding of the pathogenesis of LN. Kidney biopsies were taken from five LN patients detected LN Class II and three normal controls. The miRNA microarray chip analysis identified 66 miRNAs differentially expressed in LN. The chip results were confirmed by QRT-PCR. This work indicates that miRNAs are potential diagnosis biomarkers and probable factors involved in the pathogenesis of LN.