DEVELOPMENT OF HUMAN MONOCLONAL-ANTIBODIES TO RABIES
DEVELOPMENT OF HUMAN MONOCLONAL-ANTIBODIES TO RABIES
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DOI：
10.1089/hyb.1994.13.397
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发表时间：
1994-10-01
期刊：
影响因子：
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通讯作者：
NOTKINS, AL
中科院分区：
文献类型：
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作者：
DORFMAN, N;DIETZSCHOLD, B;NOTKINS, AL
用人二倍体细胞狂犬病疫苗免疫的人外周血B淋巴细胞，经体细胞杂交制备了9个抗狂犬病病毒的单克隆抗体。使用ELISA、Western blot和免疫沉淀法分析单克隆抗体的抗原结合特异性。不同的测定方法使得鉴定针对表面糖蛋白、核蛋白、名义磷蛋白和基质蛋白的单克隆抗体成为可能。其中一个识别表面糖蛋白的单克隆抗体中和了狂犬病毒。
A total of nine human monoclonal antibodies (MAbs) to rabies virus were generated from peripheral B lymphocytes of subjects immunized with human diploid cell rabies vaccine by somatic cell hybridization. The MAbs were analyzed for their antigen-binding specificities using ELISA, Western blot, and immunoprecipitation assays. The different assays made it possible to identify MAbs directed to the surface glycoprotein, nucleoprotein, nominal phosphoprotein, and matrix protein. One of the MAbs that recognized the surface glycoprotein neutralized rabies virus.