Identification and Characterization of microRNAs and Their Predicted Functions in Biomineralization in the Pearl Oyster (Pinctada fucata)
Identification and Characterization of microRNAs and Their Predicted Functions in Biomineralization in the Pearl Oyster (Pinctada fucata)
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DOI：
10.3390/biology8020047
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发表时间：
2019-06-01
期刊：
影响因子：
4.2
通讯作者：
Asakawa, Shuichi
中科院分区：
文献类型：
--
作者：
Huang, Songqian;Ichikawa, Yuki;Asakawa, Shuichi
珍珠形成的生物过程是一个正在进行的研究课题，并且已经确定了与此过程相关的一些基因。然而，微小RNA（miRNAs）在珍珠牡蛎，合浦珠母贝的生物矿化的参与，并没有得到很好的理解。为了研究微小RNA在合浦珠母贝中的差异和功能，我们对从闭壳肌、鳃、卵巢和外套膜组织制备的小RNA文库进行了转录组分析。我们在这些组织中鉴定了186种已知的和42种新的miRNAs。聚类分析表明，体细胞组织中miRNAs的表达模式相似，但体细胞和卵巢组织中miRNAs的表达模式存在显著差异。为了验证所鉴定的miRNA的存在，使用U6 snRNA作为内部参考，通过茎环qRT-PCR验证了9种已知的和3种新的miRNA。miRNAs和生物矿化相关基因的表达丰度和靶点预测表明，miR-1990 c-3 p、miR-876、miR-9a-3 p和novel-3可能是调控网络中的关键因子，其通过控制基质蛋白的形成或成矿细胞在外套膜组织壳形成过程中的分化发挥作用。我们的研究结果有助于进一步阐明P. fucata的生物矿化过程。
The biological process of pearl formation is an ongoing research topic, and a number of genes associated with this process have been identified. However, the involvement of microRNAs (miRNAs) in biomineralization in the pearl oyster, Pinctada fucata, is not well understood. In order to investigate the divergence and function of miRNAs in P. fucata, we performed a transcriptome analysis of small RNA libraries prepared from adductor muscle, gill, ovary, and mantle tissues. We identified 186 known and 42 novel miRNAs in these tissues. Clustering analysis showed that the expression patterns of miRNAs were similar among the somatic tissues, but they differed significantly between the somatic and ovary tissues. To validate the existence of the identified miRNAs, nine known and three novel miRNAs were verified by stem-loop qRT-PCR using U6 snRNA as an internal reference. The expression abundance and target prediction between miRNAs and biomineralization-related genes indicated that miR-1990c-3p, miR-876, miR-9a-3p, and novel-3 may be key factors in the regulatory network that act by controlling the formation of matrix proteins or the differentiation of mineralogenic cells during shell formation in mantle tissue. Our findings serve to further clarify the processes underlying biomineralization in P. fucata.