TFIIH plays an essential role in RNA polymerase I transcription
TFIIH plays an essential role in RNA polymerase I transcription
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DOI：
10.1016/s0092-8674(02)00729-8
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发表时间：
2002-05-03
期刊：
影响因子：
64.5
通讯作者：
Grummt, I
中科院分区：
文献类型：
--
作者：
Iben, S;Tschochner, H;Grummt, I
TFIIH是一种多亚基蛋白质复合物，在核苷酸切除修复和蛋白质编码基因的转录中起重要作用。在这里，我们报告TFIIH也需要在体内和体外的核糖体RNA合成。在酵母中，前rRNA合成在TFIIH ts菌株中受损。在小鼠中，部分细胞TFIIH位于核仁内，并与RNA聚合酶I和基础因子TIF-IB的亚群相关。缺乏TFIIH的转录系统是失活的，外源TFIIH恢复转录活性。TFIIH是必需的生产，但不是流产的rDNA转录，这意味着在转录后启动的作用。这些结果提供了RNA聚合酶I转录和活性核糖体RNA基因转录偶联修复之间的分子联系。
TFIIH is a multisubunit protein complex that plays an essential role in nucleotide excision repair and transcription of protein-coding genes. Here, we report that TFIIH is also required for ribosomal RNA synthesis in vivo and in vitro. In yeast, pre-rRNA synthesis is impaired in TFIIH ts strains. In a mouse, part of cellular TFIIH is localized within the nucleolus and is associated with subpopulations of both RNA polymerase I and the basal factor TIF-IB. Transcription systems lacking TFIIH are inactive and exogenous TFIIH restores transcriptional activity. TFIIH is required for productive but not abortive rDNA transcription, implying a postinitiation role in transcription. The results provide a molecular link between RNA polymerase I transcription and transcription-coupled repair of active ribosomal RNA genes.