Pre-vaccine serotype composition within a lineage signposts its serotype replacement - a carriage study over 7 years following pneumococcal conjugate vaccine use in the UK.
Pre-vaccine serotype composition within a lineage signposts its serotype replacement - a carriage study over 7 years following pneumococcal conjugate vaccine use in the UK.
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DOI：
10.1099/mgen.0.000119
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发表时间：
2017-06-30
影响因子：
3.9
通讯作者：
Clarke SC
中科院分区：
文献类型：
--
作者：
Gladstone RA;Devine V;Jones J;Cleary D;Jefferies JM;Bentley SD;Faust SN;Clarke SC
据报道，在英国和全球引入肺炎球菌结合疫苗(PCV)后，在携带者和疾病中出现了血清型替换。我们之前描述了在英国引入PCV后，与血清型替换相关的序列类型在5年内同时扩大和下降。在这里，我们使用全基因组测序来全面描述在七个冬天收集的肺炎球菌分离株的种群结构，包括在英国引入PCV7和PCV13，调查谱系在血清型替换中的重要性。我们分析了672个来自4岁或4岁以下 儿童的肺炎链球菌基因组。在血清型替换的背景下，对20个谱系的时间流行率进行了评估，这些谱系由群体结构的分层贝叶斯分析(BAPS)定义。在BAPS4、BAPS10和BAPS11三个疫苗株系中，在采样的初冬检测到多个血清型，其中有血清型替换。相反，在初冬表达单一血清型(分别为6B、6A和3)的其余三个VT谱系(BAPS1、BAPS13和BAPS14)中没有发现血清型替换。在研究结束时，在人群中没有检测到BAPS1血清6B型的谱系。在这个英国人群中，血清型替换的动态发生之前，在PCV前人群中，VT谱系中存在或不存在多种血清型。这一观察有助于预测未来在这里和其他地方引入PCV时可能涉及哪些非疫苗类型(NVT)的替代。这可能进一步表明与这些谱系相关的任何抗生素耐药性是否可能受到替换的影响。
Serotype replacement has been reported in carriage and disease after pneumococcal conjugate vaccine (PCV) introductions in the UK and globally. We previously described concurrent expansion and decline of sequence types associated with serotype replacement over 5 years following PCV introductions in the UK. Here we use whole-genome sequencing to fully characterise the population structure of pneumococcal isolates collected over seven winters encompassing PCV7 and PCV13 introductions in the UK, investigating the importance of lineages in serotype replacement. We analysed 672 pneumococcal genomes from colonised children of 4 years old or less. The temporal prevalence of 20 lineages, defined by hierarchical Bayesian analysis of population structure (BAPS), was assessed in the context of serotype replacement. Multiple serotypes were detected in the primary winter of sampling within three vaccine-type (VT) lineages BAPS4, BAPS10 and BAPS11, in which serotype replacement were observed. In contrast, serotype replacement was not seen in the remaining three VT lineages (BAPS1, BAPS13 and BAPS14), that expressed a single serotype (6B, 6A and 3, respectively) in the primary winter. One lineage, BAPS1 serotype 6B was undetectable in the population towards the end of the study period. The dynamics of serotype replacement, in this UK population, was preceded by the presence or absence of multiple serotypes within VT lineages, in the pre-PCV population. This observation could help predict which non-vaccine types (NVTs) may be involved in replacement in future PCV introductions here and elsewhere. It could further indicate whether any antibiotic resistance associated with the lineages is likely to be affected by replacement.
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影响因子：
4.5
作者：
Croucher NJ;Kagedan L;Thompson CM;Parkhill J;Bentley SD;Finkelstein JA;Lipsitch M;Hanage WP
通讯作者：
Hanage WP
影响因子：
11.8
作者：
Ladhani SN;Slack MP;Andrews NJ;Waight PA;Borrow R;Miller E
通讯作者：
Miller E
影响因子：
6.7
作者：
Brueggemann, Angela B.;Pai, Rekha;Beall, Bernard
通讯作者：
Beall, Bernard
影响因子：
5.5
作者：
Devine, Vanessa T.;Cleary, David W.;Clarke, Stuart C.
通讯作者：
Clarke, Stuart C.
影响因子：
3.6
作者：
Farrell, David J.;Klugman, Keith P.;Pichichero, Michael
通讯作者：
Pichichero, Michael