Intracapillary HbO2 saturations in murine tumours and human tumour xenografts measured by cryospectrophotometry: relationship to tumour volume, tumour pH and fraction of radiobiologically hypoxic cells.
Intracapillary HbO2 saturations in murine tumours and human tumour xenografts measured by cryospectrophotometry: relationship to tumour volume, tumour pH and fraction of radiobiologically hypoxic cells.
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通过冷冻分光光度法测量小鼠肿瘤和人类肿瘤异种移植物中的毛细血管内 HbO2 饱和度：与肿瘤体积、肿瘤 pH 值和放射生物学低氧细胞分数的关系。
DOI：
10.1038/bjc.1988.113
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发表时间：
1988
影响因子：
8.8
通讯作者：
Sutherland,RM
中科院分区：
文献类型：
--
作者：
Rofstad,EK;Fenton,BM;Sutherland,RM
使用冷冻分光光度法测定了两种小鼠肿瘤细胞系（KHT，RIF-1）和两种人卵巢癌异种移植细胞系（MLS，OWI）的毛细血管内HbO 2饱和度的频率分布。目的是寻找HbO 2饱和状态和肿瘤体积，肿瘤pH值和放射性缺氧细胞分数之间的可能关系。通过31 P NMR光谱测量肿瘤pH。从体内照射的肿瘤的细胞存活曲线和体外测定中确定低血糖分数。研究了体积范围为100-4000 mm 3的肿瘤，发现大多数血管的HbO 2饱和度低于10%。四种肿瘤细胞系之间的HbO 2频率分布的体积依赖性显著不同; KHT、RIF-1和MLS细胞系的HbO 2饱和状态随肿瘤体积的增加而降低，并且与OWI细胞系的肿瘤体积无关。数据表明，在肿瘤生长过程中，HbO 2饱和状态的下降速率与坏死的发展速率有关。肿瘤pH值的体积依赖性与所有肿瘤细胞系的HbO 2饱和状态非常相似。因此，显着的相关性，发现之间的HbO 2饱和状态和肿瘤pH值，无论是在肿瘤线和跨四个肿瘤线，反映这两个参数的体积依赖性可能是一个强制性的结果，减少肿瘤生长过程中的供氧条件。低氧分数增加肿瘤生长过程中的KHT，RIF-1和MLS线和体积无关的OWI线，表明肿瘤细胞系内的HbO 2饱和状态和低氧分数之间的关系。然而，这两个参数之间没有相关性在四个肿瘤线，表明肿瘤的缺氧分数不仅由氧气供应条件决定;其他参数也可能是重要的，如氧扩散率，氧消耗率和细胞在缺氧应激下的存活时间。
Frequency distributions for intracapillary HbO2 saturation were determined for two murine tumour lines (KHT, RIF-1) and two human ovarian carcinoma xenograft lines (MLS, OWI) using a cryospectrophotometric method. The aim was to search for possible relationships between HbO2 saturation status and tumour volume, tumour pH and fraction of radiobiologically hypoxic cells. Tumour pH was measured by 31P NMR spectroscopy. Hypoxic fractions were determined from cell survival curves for tumours irradiated in vivo and assayed in vitro. Tumours in the volume range 100-4000 mm3 were studied and the majority of the vessels were found to have HbO2 saturations below 10%. The volume-dependence of the HbO2 frequency distributions differed significantly among the four tumour lines; HbO2 saturation status decreased with increasing tumour volume for the KHT, RIF-1 and MLS lines and was independent of tumour volume for the OWI line. The data indicated that the rate of decrease in HbO2 saturation status during tumour growth was related to the rate of development of necrosis. The volume-dependence of tumour pH was very similar to that of the HbO2 saturation status for all tumour lines. Significant correlations were therefore found between HbO2 saturation status and tumour pH, both within tumour lines and across the four tumour lines, reflecting that the volume-dependence of both parameters probably was a compulsory consequence of reduced oxygen supply conditions during tumour growth. Hypoxic fraction increased during tumour growth for the KHT, RIF-1 and MLS lines and was volume-independent for the OWI line, suggesting a relationship between HbO2 saturation status and hypoxic fraction within tumour lines. However, there was no correlation between these two parameters across the four tumour lines, indicating that the hypoxic fraction of a tumour is not determined only by the oxygen supply conditions; other parameters may also be important, eg oxygen diffusivity, rate of oxygen consumption and cell survival time under hypoxic stress.