BK channels regulate calcium oscillations in ventricular myocytes on different substrate stiffness.
BK channels regulate calcium oscillations in ventricular myocytes on different substrate stiffness.
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DOI：
10.1016/j.lfs.2019.116802
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发表时间：
2019-10
期刊：
影响因子：
6.1
通讯作者：
Sisi Liu;Xiaohui Gao;Xiaoan Wu;Yang Yu;Zhang Yu;Sui Zhao;Hucheng Zhao
中科院分区：
文献类型：
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作者：
Sisi Liu;Xiaohui Gao;Xiaoan Wu;Yang Yu;Zhang Yu;Sui Zhao;Hucheng Zhao
基质硬度对细胞功能至关重要，但细胞感知机械信号的机制仍不清楚。在这里，我们表明，频率和幅度的自发钙振荡的鸡心肌细胞培养的硬基板上比软基板。自发的Ca ~（2+）振荡增加刚性基板。然而，一个消除依赖的Ca 2+振荡基板刚度后，观察到大电导Ca 2+激活的K+（BK）通道的阻滞剂。此外，培养在刚性基质上的心肌细胞的BK通道活性降低。这些结果提供了令人信服的证据表明，BK通道是至关重要的底物刚度依赖性调节的钙振荡的心肌细胞。
Substrate stiffness is essential for cell functions, but the mechanisms by which cell sense mechanical cues are still unclear. Here we show that the frequency and the amplitude of spontaneous Ca2+oscillations were greater in chick cardiomyocytes cultured on the stiff substrates than that on the soft substrates. The spontaneous Ca2+oscillations were increased on stiff substrates. However, an eliminated dependence of the Ca2+oscillations on substrate stiffness was observed after applying blocker of the large-conductance Ca2+-activated K+(BK) channels. In addition, the activity of BK channels in cardiomyocytes cultured on the stiff substrates was decreased. These results provide compelling evidences to show that BK channels are crucial in substrate stiffness-dependent regulation of the Ca2+oscillation in cardiomyocytes.