Performance of a Biodegradable Composite with Hydroxyapatite as a Scaffold in Pulp Tissue Repair
Performance of a Biodegradable Composite with Hydroxyapatite as a Scaffold in Pulp Tissue Repair
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DOI：
10.3390/polym12040937
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发表时间：
2020-04-01
期刊：
影响因子：
5
通讯作者：
Hayashi, Mikako
中科院分区：
文献类型：
--
作者：
Okamoto, Motoki;Matsumoto, Sayako;Hayashi, Mikako
活髓治疗是一种重要的根管治疗方法。使用生长因子和生物分子的策略在开发基于牙本质-牙髓复合体伤口愈合的盖髓材料方面是有效的。我们团队开发了用于组织工程医疗设备的可生物降解的粘弹性聚合物材料。聚合物成分有助于克服羟基磷灰石(HAP)促进牙髓细胞成骨分化的断裂韧性差。然而，这种新型聚合物的组成仍然不清楚。本研究评价了一种新型聚合物复合材料P(CL-co-D11a)和羟基磷灰石(HAP)作为生物分子直接盖髓载体的作用。通过测定体外培养的人牙科干细胞的细胞毒性和增殖能力，评价新型聚合物复合材料的生物相容性。据报道，这种含有BMP-2的新型聚合物复合材料可以诱导第三牙本质，作为一种直接的盖髓材料在大鼠模型上进行了测试。细胞毒性和细胞增殖实验表明，新型聚合物复合材料的生物相容性与对照组相似。在直接盖髓模型中，新型聚合物复合材料与BMP-2诱导的第三级牙本质相似，类似于水硬性硅酸钙骨水泥。与单独的新型聚合物复合材料相比，BMP-2复合材料上调了伤口愈合相关基因的表达。因此，我们认为新型聚合物复合材料可以作为盖髓的有效载体。
Vital pulp therapy is an important endodontic treatment. Strategies using growth factors and biological molecules are effective in developing pulp capping materials based on wound healing by the dentin-pulp complex. Our group developed biodegradable viscoelastic polymer materials for tissue-engineered medical devices. The polymer contents help overcome the poor fracture toughness of hydroxyapatite (HAp)-facilitated osteogenic differentiation of pulp cells. However, the composition of this novel polymer remained unclear. This study evaluated a novel polymer composite, P(CL-co-DLLA) and HAp, as a direct pulp capping carrier for biological molecules. The biocompatibility of the novel polymer composite was evaluated by determining the cytotoxicity and proliferation of human dental stem cells in vitro. The novel polymer composite with BMP-2, which reportedly induced tertiary dentin, was tested as a direct pulp capping material in a rat model. Cytotoxicity and proliferation assays revealed that the biocompatibility of the novel polymer composite was similar to that of the control. The novel polymer composite with BMP-2-induced tertiary dentin, similar to hydraulic calcium-silicate cement, in the direct pulp capping model. The BMP-2 composite upregulated wound healing-related gene expression compared to the novel polymer composite alone. Therefore, we suggest that novel polymer composites could be effective carriers for pulp capping.