Comparative analysis of human and mouse 3' Igh regulatory regions identifies distinctive structural features.
Comparative analysis of human and mouse 3' Igh regulatory regions identifies distinctive structural features.
复制标题
对人和小鼠 3 Igh 调节区的比较分析确定了独特的结构特征。
DOI：
10.1016/j.molimm.2004.09.006
复制
发表时间：
2005
影响因子：
3.6
通讯作者：
Birshtein,BarbaraK
中科院分区：
文献类型：
--
作者：
Sepulveda,ManuelA;Garrett,FrancineE;Price-Whelan,Alexa;Birshtein,BarbaraK
Immunoglobulin heavy chain (Igh) locus rearrangements are controlled in part by an ∼30kb complex 3′ regulatory region located 3′ of Cα: this region contains several enhancers. We report here the comparison of the genomic sequences of the 3′ regulatory region and further downstream sequences from mouse, rat, human and chimpanzee. Only short segments of homology were detected in the 3′ regulatory region, and these were located in the vicinity of the known 3′ enhancers. The nearest highly conserved segment is the nearest non-Igh gene, hole, which is located ∼62kb downstream of mouse Cα. Analysis of murine 3′Igh sequences by single nucleotide polymorphism (SNP) and restriction fragment length polymorphism (RFLP) detected a transition region (high to low SNP or RFLP density) ∼120kb downstream of mouse Cα. Although there is only limited sequence identity between rodent and primate 3′Igh regulatory regions, all of these regulatory regions contain a palindrome and locally repetitive elements. Locally repetitive elements in primates comprise blocks of “switch-like” sequences that differ from the families of inverted and tandem repeats that are present in rodents. We propose that together with enhancers, these “conserved” structural features are essential for the activity of the 3′Igh regulatory region in vivo.
