Both RIG-I and MDA5 detect alphavirus replication in concentration-dependent mode.
Both RIG-I and MDA5 detect alphavirus replication in concentration-dependent mode.
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DOI：
10.1016/j.virol.2015.09.023
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发表时间：
2016-01
期刊：
影响因子：
3.7
通讯作者：
Frolova EI
中科院分区：
文献类型：
--
作者：
Akhrymuk I;Frolov I;Frolova EI
Alphaviruses are a family of positive-strand RNA viruses that circulate on all continents between mosquito vectors and vertebrate hosts. Despite a significant public health threat, their biology is not sufficiently investigated, and the mechanisms of alphavirus replication and virus-host interaction are insufficiently understood. In this study, we have applied a variety of experimental systems to further understand the mechanism by which infected cells detect replicating alphaviruses. Our new data strongly suggest that activation of the antiviral response by alphavirus-infected cells is determined by the integrity of viral genes encoding proteins with nuclear functions, and by the presence of two cellular pattern recognition receptors (PRRs), RIG-I and MDA5. No type I IFN response is induced in their absence. The presence of either of these PRRs is sufficient for detecting virus replication. However, type I IFN activation in response to pathogenic alphaviruses depends on the basal levels of RIG-I or MDA5.
