Molecular Characterization of Carbonic Anhydrase Genes in Lotus japonicus and Their Potential Roles in Symbiotic Nitrogen Fixation.
Molecular Characterization of Carbonic Anhydrase Genes in Lotus japonicus and Their Potential Roles in Symbiotic Nitrogen Fixation.
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莲藕碳酸酐酶基因的分子特征及其在共生固氮中的潜在作用
DOI：
10.3390/ijms22157766
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发表时间：
2021-07-21
影响因子：
5.6
通讯作者：
Duanmu D
中科院分区：
文献类型：
--
作者：
Wang L;Liang J;Zhou Y;Tian T;Zhang B;Duanmu D
碳酸酐酶（CA）在高等植物的光合组织中起着重要作用，而在共生根瘤中的非光合作用却很少被研究。本研究通过与拟南芥CA基因的比较，在模式豆科植物百脉根中鉴定了13个CA基因。利用qPCR和启动子-报告基因融合方法，进一步表征了三个先前鉴定的结节增强CA基因（Ljα CA 2、Ljα CA 6和Ljβ CA 1），它们在结节发育期间表现出不同的时空表达模式。Ljα CA 2在成熟结节的中央感染区表达，包括感染和未感染的细胞。Ljα CA 6主要定位于根和根瘤的维管束中。Ljβ CA 1在结瘤原基的大多数细胞类型中都有表达，但仅在成熟结瘤的外周皮层细胞和未感染细胞中有表达。使用CRISPR/Cas9技术，与野生型植物相比，Ljβ CA 1或Ljα CA 2及其同源物Ljα CA 1的敲除未导致异常共生表型，表明Ljβ CA 1或Ljα CA 1/2对于正常共生条件下的固氮不是必需的。然而，根瘤增强的表达模式和不同类型的细胞中的不同分布暗示了它们在根瘤共生过程中的潜在功能，如CO2固定，N同化和pH调节，有待进一步研究。
Carbonic anhydrase (CA) plays a vital role in photosynthetic tissues of higher plants, whereas its non-photosynthetic role in the symbiotic root nodule was rarely characterized. In this study, 13 CA genes were identified in the model legume Lotus japonicus by comparison with Arabidopsis CA genes. Using qPCR and promoter-reporter fusion methods, three previously identified nodule-enhanced CA genes (LjαCA2, LjαCA6, and LjβCA1) have been further characterized, which exhibit different spatiotemporal expression patterns during nodule development. LjαCA2 was expressed in the central infection zone of the mature nodule, including both infected and uninfected cells. LjαCA6 was restricted to the vascular bundle of the root and nodule. As for LjβCA1, it was expressed in most cell types of nodule primordia but only in peripheral cortical cells and uninfected cells of the mature nodule. Using CRISPR/Cas9 technology, the knockout of LjβCA1 or both LjαCA2 and its homolog, LjαCA1, did not result in abnormal symbiotic phenotype compared with the wild-type plants, suggesting that LjβCA1 or LjαCA1/2 are not essential for the nitrogen fixation under normal symbiotic conditions. Nevertheless, the nodule-enhanced expression patterns and the diverse distributions in different types of cells imply their potential functions during root nodule symbiosis, such as CO2 fixation, N assimilation, and pH regulation, which await further investigations.
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影响因子：
64.8
作者：
Engineer, Cawas B.;Ghassemian, Majid;Anderson, Jeffrey C.;Peck, Scott C.;Hu, Honghong;Schroeder, Julian I.
通讯作者：
Schroeder, Julian I.
影响因子：
7.4
作者：
KING, BJ;LAYZELL, DB;CANVIN, DT
通讯作者：
CANVIN, DT
影响因子：
7.4
作者：
Gálvez, S;Hirsch, AM;Crespi, M
通讯作者：
Crespi, M
影响因子：
11.6
作者：
Huang, Jian;Li, Zhiyong;Zhao, Dazhong
通讯作者：
Zhao, Dazhong
影响因子：
6.9
作者：
Fischinger SA;Hristozkova M;Mainassara ZA;Schulze J
通讯作者：
Schulze J