The differential adhesion hypothesis: a direct evaluation
The differential adhesion hypothesis: a direct evaluation
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DOI：
10.1016/j.ydbio.2004.11.012
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发表时间：
2005-02-01
影响因子：
2.7
通讯作者：
Steinberg, MS
中科院分区：
文献类型：
--
作者：
Foty, RA;Steinberg, MS
20世纪60年代提出的差异粘附假说（DAH）认为，发育过程中的液体状组织铺展和细胞分离现象是由组织表面张力引起的，而组织表面张力又是由细胞间粘附的差异引起的。我们早期对液体状细胞聚集体表面张力的测量表明，无一例外地，表面张力较低的细胞聚集体倾向于包裹它所粘附的表面张力较高的细胞聚集体。在这里，我们测量的表面张力L细胞聚集体转染表达N-，P-或E-钙粘蛋白在不同的，测量量。我们报告说，在这些聚集体中，其中钙粘蛋白基本上是唯一的细胞-细胞粘附分子，聚集体表面张力是钙粘蛋白表达水平的直接线性函数。结合我们早期的结果，得出的结论是，液体状的形态发生细胞和组织重排的细胞分选，组织扩散和分离代表自组装过程的指导下减少的粘附自由能的细胞往往最大限度地提高他们的相互结合。这一结论涉及的物理学管理这些形态发生现象，并适用于独立的问题，如细胞间的粘合剂的特异性。(C)2004年爱思唯尔公司All rights reserved.
The differential adhesion hypothesis (DAH), advanced in the 1960s, proposed that the liquid-like tissue-spreading and cell segregation phenomena of development arise from tissue surface tensions that in turn arise from differences in intercellular adhesiveness. Our earlier measurements of liquid-like cell aggregate surface tensions have shown that, without exception, a cell aggregate of lower surface tension tends to envelop one of higher surface tension to which it adheres. We here measure the surface tensions of L cell aggregates transfected to express N-, P- or E-cadherin in varied, measured amounts. We report that in these aggregates, in which cadherins are essentially the only cell-cell adhesion molecules, the aggregate surface tensions are a direct, linear function of cadherin expression level. Taken together with our earlier results, the conclusion follows that the liquid-like morphogenetic cell and tissue rearrangements of cell sorting, tissue spreading and segregation represent self-assembly processes guided by the diminution of adhesive-free energy as cells tend to maximize their mutual binding. This conclusion relates to the physics governing these morphogenetic phenomena and applies independently of issues such as the specificities of intercellular adhesives. (C) 2004 Elsevier Inc. All rights reserved.