Isolation and characterisation of a MADS-box gene in cucumber (Cucumis sativus L.) that affects flowering time and leaf morphology in transgenic Arabidopsis
Isolation and characterisation of a MADS-box gene in cucumber (Cucumis sativus L.) that affects flowering time and leaf morphology in transgenic Arabidopsis
复制标题
黄瓜 (Cucumis sativus L.) 中影响转基因拟南芥开花时间和叶片形态的 MADS-box 基因的分离和表征
DOI：
10.1080/13102818.2018
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发表时间：
--
影响因子：
1.4
通讯作者：
Shiqiang Liu
中科院分区：
文献类型：
--
作者：
Yong Zhou;Lifang Hu;Jianbo Song;Lunwei Jiang;Shiqiang Liu
MADS-box家族基因是重要的转录因子，在植物生长和发育中发挥重要作用。在这项研究中，在黄瓜 (Cucumis sativus L.) 中鉴定了 MADS-box 基因 CsMADS02 并进行了功能表征。 CsMADS02编码典型的SEP MADS-.box蛋白，其包含MADS-box、K-box、I区和C区以及两个短保守SEP基序。序列比对和系统发育分析表明，CsMADS02蛋白属于SEP3亚家族，与其他已发表的植物SEP蛋白高度同源。表达分析表明，CsMADS02主要在花和子房中表达，并且在雄花和雌花发育过程中差异表达。 CsMADS02 的异位过度表达导致拟南芥提前开花。此外，在转基因植物中也观察到形态变化，包括卷曲和较少的莲座状叶子。这些发现表明 CsMADS02 是黄瓜开花时间和叶片形态的潜在调节剂
The MADS-box family genes are important transcription factors that play essential roles in plant.growth and development. In this study, a MADS-box gene, CsMADS02, was identified and func-.tionally characterized in cucumber (Cucumis sativus L.). CsMADS02 encoded a typical SEP MADS-.box protein which contained a MADS-box, a K-box, an I region and a C region, as well as two.short conserved SEP motifs. Sequence alignment and phylogenetic analysis showed that the.CsMADS02 protein belonged to the SEP3 subfamily and was highly homologous to other pub-.lished plant SEP proteins. Expression analyses revealed that CsMADS02 was primarily expressed.in flowers and ovaries, and differentially expressed during the development of male and female.flowers. Ectopic overexpression of CsMADS02 caused earlier flowering of Arabidopsis. In addition,.morphological changes were also observed in transgenic plants, including curled and fewer ros-.ette leaves. These findings suggest that CsMADS02 is a potential regulator of the flowering time.and leaf morphology in cucumber