Preparation and bioactivity of acetylated konjac glucomannan fibrous membrane and its application for wound dressing.
Preparation and bioactivity of acetylated konjac glucomannan fibrous membrane and its application for wound dressing.
复制标题
DOI：
10.1016/j.carbpol.2019.115404
复制
发表时间：
2020-02
影响因子：
11.2
通讯作者：
Chuang Wang;Bing Li;Tao Chen;Naibin Mei;Xiaoying Wang;Shunqing Tang
中科院分区：
文献类型：
--
作者：
Chuang Wang;Bing Li;Tao Chen;Naibin Mei;Xiaoying Wang;Shunqing Tang
生物材料-宿主的相互作用显著影响组织修复，而组织修复是由巨噬细胞调节的。本研究采用不同取代度(DS)对魔芋葡甘聚糖(KGM)进行乙酰化，并设计了基于乙酰化KGM(AceKGM)的纤维膜，以调节巨噬细胞的活性以促进创面愈合。AceKGM具有生物相容性，易溶于有机溶剂。用原子力显微镜(AFM)定量检测Raw264.7细胞与AceKGM底物之间的粘附力。酶联免疫吸附试验结果显示，AceKGM纤维膜可增强巨噬细胞抗炎和促再生细胞因子的表达，且其DS对膜的生物活性有显著影响。全层小鼠皮肤创面修复实验表明，含有AceKGM的纤维膜通过促进再上皮化、组织重塑和胶原沉积而显著促进创面愈合。综上所述，基于AceKGM的纤维膜具有作为创面再生的生物活性支架的潜力。
Biomaterial-host interactions significantly affect tissue repair, which is modulated by macrophages. In this study, a polysaccharide, konjac glucomannan (KGM), was acetylated with different degrees of substitution (DS), and the acetylated KGM (AceKGM)-based fibrous membrane was designed to modulate the activity of macrophages for accelerating wound healing. AceKGM was biocompatible and easily dissolved in organic solvents. The adhesion force between Raw264.7 cells and the AceKGM substrate was quantitatively detected by atomic force microscopy (AFM). The enzyme-linked immunosorbent assay (ELISA) results showed that the AceKGM fibrous membrane enhanced macrophage expression of anti-inflammatory and pro-regenerative cytokines, and the DS of AceKGM significantly affected membrane bioactivity. The full-thickness mouse skin wound repair experiments indicated that the AceKGM-containing fibrous membranes significantly accelerated wound healing by promoting re-epithelialization, tissue remodeling, and collagen deposition. In summary, AceKGM-based fibrous membranes have potential as bioactive scaffolds for wound regeneration.