Clinical coxsackievirus B isolates differ from laboratory strains in their interaction with two cell surface receptors
Clinical coxsackievirus B isolates differ from laboratory strains in their interaction with two cell surface receptors
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DOI：
10.1093/infdis/175.3.697
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发表时间：
1997-03-01
影响因子：
6.4
通讯作者：
Finberg, RW
中科院分区：
文献类型：
--
作者：
Bergelson, JM;Modlin, JF;Finberg, RW
柯萨奇 B 病毒与两种假定的细胞表面受体分子相互作用。对原型实验室菌株的实验表明，所有 6 种柯萨奇 B 血清型均与单克隆抗体 RmcB 识别的 46 kDa 蛋白质相互作用，而 CB1、CB3 和 CB5 也可能与腐烂加速因子结合。抗受体单克隆抗体用于研究低传代临床柯萨奇 B 病毒分离株与两种受体之间的相互作用。与单一原型菌株的观察结果相反，这些数据表明临床分离株的受体使用与血清型并不严格相关，甚至具有不同传代历史的原型菌株的受体使用也可能不同。在给定的血清型中，各个病毒分离株与特定受体结合的能力存在差异，并且在人类和组织培养中感染期间可能会出现受体特异性改变的变体。
Coxsackie B viruses interact with two putative cell surface receptor molecules. Experiments with prototype laboratory strains suggest that all 6 coxsackie B serotypes interact with a 46-kDa protein recognized by the monoclonal antibody RmcB, whereas CB1, CB3, and CB5 may also bind to decay accelerating factor. Antireceptor monoclonal antibodies were used to study interactions between low-passage clinical coxsackie B virus isolates and the two receptors. In contrast to observations made with single prototype strains, these data indicate that receptor use by clinical isolates is not strictly related to serotype and that even prototype strains with different passage histories may differ in receptor use. Within a given serotype, variation exists in the capacity of individual virus isolates to bind to specific receptors, and variants with altered receptor specificity may arise during infection in humans and in tissue culture.