Nucleosomes contain DNA binding proteins that resist dissociation by sodium dodecyl sulfate.
Nucleosomes contain DNA binding proteins that resist dissociation by sodium dodecyl sulfate.
复制标题
核小体含有 DNA 结合蛋白，可抵抗十二烷基硫酸钠的解离。
DOI：
10.1016/0006-291x(83)91599-1
复制
发表时间：
1983
影响因子：
3.1
通讯作者：
Muller,MT
中科院分区：
文献类型：
--
作者：
Muller,MT
一种新的、快速的、定量的方法已经被开发出来，以证明核小体含有非组蛋白，这些蛋白不会被离子去污剂解离，并且牢固地（可能是共价地）与DNA结合。该方法能够检测和定量已知与 DNA 形成稳定（共价）复合物的蛋白质的结合，已使用纯化的拓扑异构酶 I 进行了验证；此外，该方法还将测量紫外线形成的DNA/蛋白质加合物。去污剂抗性蛋白在核小体群体中的分布是不均匀的；单聚体到三聚体的这些蛋白质被耗尽，而高级寡聚体则被富集。数据表明这些蛋白质与核小体核心无关，但更可能与核心颗粒之间的连接区域相关。
A new, rapid, and quantitative method has been developed to show that nucleosomes contain non-histone proteins which are not dissociated by an ionic detergent and are firmly, possibly covalently, bound to DNA. The ability of the method to detect and quantitate the binding of proteins known to form stable (covalent) complexes with DNA has been verified using purified topoisomerase I; in addition, the method will measure DNA/protein adducts formed by ultraviolet light. The distribution of detergent resistant proteins in a population of nucleosomes is non-uniform; the mono- through trimers are depleted of these proteins while higher oligomers are enriched. The data suggest that these proteins are not associated with the nucleosome core but more likely with linker regions between core particles.