Vesicular transport of horseradish peroxidase from brain to blood in segments of the cerebral microvasculature in adult mice
Vesicular transport of horseradish peroxidase from brain to blood in segments of the cerebral microvasculature in adult mice
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成年小鼠大脑微血管部分中辣根过氧化物酶从大脑到血液的囊泡转运
DOI：
10.1016/0006-8993(77)90859-9
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发表时间：
1977
期刊：
影响因子：
2.9
通讯作者：
B. Deurs
中科院分区：
文献类型：
--
作者：
B. Deurs
用电子显微镜观察了小鼠脑室蛋白质向脑实质血流的转移。在用蛋白示踪剂辣根过氧化物酶（HRP; M.W.约40，000）穿过脑室，示踪剂穿透脑室的室管膜衬里，并在靠近室管膜的神经细胞的细胞外间隙中发现。HRP也见于血管基底膜和在近腔内皮表面开口的内皮囊泡中，或位于微血管（主要是小动脉）段的内皮细胞内。在这些节段中的一些，HRP也被认为是在管腔表面的内皮细胞和表面连接的囊泡。连接相邻内皮细胞的连接处从未被HRP穿透。它的结论是，囊泡运输HRP跨脑微血管内皮细胞的蛋白质从脑细胞外空间的清除代表了一种可能的机制。
The transfer of protein from the cerebral ventricles to the parenchymal blood-stream in mice was studied by electron microflapy. After perfusion with the protein tracer horseradish peroxidase (HRP; M.W. approx. 40,000) through the cerebral ventricles, the tracer penetrated the ependymal lining of the ventricles and was found in the extracellular space of the neuropil close to the ependyma. HRP was also seen in the vascular basement membrane and in endothelial vesicles opening at the abluminal endothelial surface, or situated within the endothelial cells in segments of the microvasculature (mostly small arterioles). In some of these segments HRP was also seen on the luminal surface of the endothelia and in surface-connected vesicles. The junctions connecting adjacent endothelial cellls were never penetrated by HRP. It is concluded that vesicular transport of HRP across the endothelium of the cerebral microvasculature represents a possible mechanism for protein removal from brain extracellular space.