Isolation and characterization of cDNAs encoding an enzyme with glucosyltransferase activity for cyclo-DOPA from four o'clocks and feather cockscombs
Isolation and characterization of cDNAs encoding an enzyme with glucosyltransferase activity for cyclo-DOPA from four o'clocks and feather cockscombs
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DOI：
10.1093/pcp/pci064
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发表时间：
2005-04-01
影响因子：
4.9
通讯作者：
Ozeki, Y
中科院分区：
文献类型：
--
作者：
Sasaki, N;Wada, K;Ozeki, Y
编码具有UDP-葡萄糖：环-DOPA 5-O-葡萄糖基转移酶活性的酶的cDNA从四点钟和羽毛鸡冠中分离。从cDNA推导的氨基酸序列的系统发育分析表明，它们代表一个单一的亚支，不同于其他苯丙素和类黄酮葡萄糖基转移酶。在四点钟的cDNA的转录物的量的变化与甜菜苷的积累。在花的发育过程中。这里分离的cDNA是参与另一种甜菜花青苷生物合成途径的酶基因的候选基因，该途径通过环多巴步骤而不是甜菜苷步骤的糖基化进行。
cDNAs encoding an enzyme with UDP-glucose:cyclo-DOPA 5-O-glucosyltransferase activity were isolated from four o'clocks and feather cockscombs. Phylogenetic analysis of the amino acid sequences deduced from the cDNAs show that they represent a single subclade distinct from those of other phenylpropanoid and flavonoid glucosyltransferases. Changes in the amount of transcripts of the cDNA in four o'clocks correlated with the accumulation of betanin. during flower development. The cDNAs isolated here were candidates for the gene of the enzyme involved in another pathway of betacyanin biosynthesis via glucosylation at the cyclo-DOPA step rather than at the betanidin step.