Purified bovine AMH induces a characteristic freemartin effect in fetal rat prospective ovaries exposed to it in vitro.
Purified bovine AMH induces a characteristic freemartin effect in fetal rat prospective ovaries exposed to it in vitro.
复制标题
纯化的牛 AMH 在体外暴露于其的胎鼠预期卵巢中诱导特征性的 freemartin 效应。
作者：
B. Vigier;Françoise Watrin;Solange Magre;D. Tran;N. Josso
为了确定抗苗勒氏管激素 (AMH) 是否是在通过胎盘吻合术与雄性双胞胎 (freemartins) 结合的牛遗传女性中观察到的性腺损伤的原因，将胎鼠的未来卵巢暴露于体外纯化的牛 AMH。在 14 天开始的培养中并维持 3 至 10 天，AMH 持续诱导特有的“弗里马丁效应”，即性腺体积减少、生殖细胞耗竭以及性腺胚基中上皮细胞的分化，这些细胞具有通过叉指连接的大而透明的细胞质，类似于大鼠胎儿支持细胞。这些细胞倾向于极化并形成索，由含有层粘连蛋白和纤连蛋白的连续基底膜描绘。这种结构类似于发育中的生精索，在对照卵巢培养物中没有检测到。这些数据强烈表明，AMH 是引发 freemartin 性腺形态异常的睾丸因素。
To determine whether anti-Müllerian hormone (AMH) is responsible for the gonadal lesions observed in bovine genetic females united by placental anastomoses to male twins (freemartins), prospective ovaries of fetal rats were exposed to purified bovine AMH in vitro. In cultures initiated at 14 days p.c. and maintained 3 to 10 days, AMH consistently induced a characteristic 'freemartin effect', namely reduction of gonadal volume, germ cell depletion and differentiation, in the gonadal blastema, of epithelial cells with large clear cytoplasm linked by interdigitations, resembling rat fetal Sertoli cells. These cells tend to become polarized and form cords, delineated by a continuous basal membrane containing laminin and fibronectin. Such structures, resembling developing seminiferous cords, were not detected in control ovarian cultures. These data strongly suggest that AMH is the testicular factor responsible for triggering the morphological abnormalities of freemartin gonads.