Complete digestion/biodegradation of polystyrene microplastics by greater wax moth (Galleria mellonella) larvae: Direct in vivo evidence, gut microbiota independence, and potential metabolic pathways.
Complete digestion/biodegradation of polystyrene microplastics by greater wax moth (Galleria mellonella) larvae: Direct in vivo evidence, gut microbiota independence, and potential metabolic pathways.
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DOI：
10.1016/j.jhazmat.2021.127213
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发表时间：
2022-02
影响因子：
13.6
通讯作者：
Shuai Wang;Wei Shi;Zhichu Huang;Nihong Zhou;Yanling Xie;Yu Tang;F. Hu;Guangxu Liu;Huoqing Zheng
中科院分区：
文献类型：
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作者：
Shuai Wang;Wei Shi;Zhichu Huang;Nihong Zhou;Yanling Xie;Yu Tang;F. Hu;Guangxu Liu;Huoqing Zheng
食塑料昆虫如大蜡蛾(Galleria Mellonella)对塑料聚合物的生物降解可能在减少塑料污染方面有希望，但直接的活体证据以及相关的肠道微生物区系的代谢途径和作用需要进一步的研究。在这项研究中，我们研究了在活体降解过程中，潜在的代谢途径，以及肠道微生物区系在聚苯乙烯(PS)生物降解中的参与。Mellonalarvae(天津，中国)，含有PS微珠(0.5 mg/头；MN：540，MW：550)和通用PS粉(2.5 mg/头；MN：95,600，Mw：217,000)。结果表明，PS微塑料的解聚和完全消化不依赖于肠道微生物菌群。肠道微生物区系能促进代谢。在比较代谢组学和高效液相分析的基础上，我们提出了PS在OFG肠道中的两条可能的代谢途径。Mellonalarvae：环氧苯乙醛和4-methylphenol–4-hydroxybenzaldehyde–4-hydroxybenzoate途径。这些结果表明，G。菌丝体对PS的高效生物降解起着重要作用。需要进一步的研究来鉴定这些酶并研究其潜在的催化机制。
Biodegradation of plastic polymers by plastic-eating insects such as the greater wax moth (Galleria mellonella) might be promising for reducing plastic pollution, but directin vivoevidence along with the related metabolic pathways and role of gut microbiota require further investigation. In this study, we investigated thein vivodegradation process, underlying potential metabolic pathways, and involvement of the gut microbiota in polystyrene (PS) biodegradation via enforcing injection ofG. mellonellalarvae (Tianjin, China) with PS microbeads (0.5 mg/larva; Mn: 540 and Mw: 550) and general-purpose PS powders (2.5 mg/larva; Mn: 95,600 and Mw: 217,000). The results indicated that the PS microplastics were depolymerized and completely digested independent of gut microbiota inG. mellonellaalthough the metabolism could be enhanced by gut microbiota. Based on comparative metabolomic and liquid chromatography analyses, we proposed two potential metabolic pathways of PS in the intestine ofG. mellonellalarvae: the styrene oxide–phenylacetaldehyde and 4-methylphenol–4-hydroxybenzaldehyde–4-hydroxybenzoate pathways. These results suggest that the enzymes ofG. mellonellaare responsible for the efficient biodegradation of PS. Further study is needed to identify these enzymes and investigate the underlying catalytic mechanisms.