LARGE CHANGE IN AXON FLUORESCENCE THAT PROVIDES A PROMISING METHOD FOR MEASURING MEMBRANE-POTENTIAL
LARGE CHANGE IN AXON FLUORESCENCE THAT PROVIDES A PROMISING METHOD FOR MEASURING MEMBRANE-POTENTIAL
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DOI：
10.1038/newbio241159a0
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发表时间：
1973-01-01
期刊：
影响因子：
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通讯作者：
WAGGONER, AS
中科院分区：
文献类型：
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作者：
DAVILA, HV;SALZBERG, BM;WAGGONER, AS
为了理解动作电位传播的分子和结构基础，已经对动作电位过程中轴突的双折射1、光散射1和外源性荧光2的变化进行了详细的研究3 - 5。这些光学变化在另一种情况下也可能是有用的。由于许多变化与动作电位发生的时间过程相同，因此理论上可以使用光学技术来测量膜电位。先前描述的光学变化相对较小，并且为了测量单个轴突的变化，必须对许多试验的结果进行平均。我们已经尝试了100多种荧光染料，当我们注意到mercury染料时，最好的一种在巨大轴突的动作电位中给出了2：1的信噪比。这些染料中的一种给出了相当大的信号，在单次扫描中很容易看到。这种荧光变化可以提供一个强大的工具，用于测量膜电位的系统中，使用电极是不方便或不可能的。
THE changes in birefringence1, light scattering1, and extrinsic fluorescence2of axons that occur during the action potential have been studied in some detail3–5in an attempt to understand the molecular and structural basis of action potential propagation. These optical changes could also be useful in another context. Because many of the changes occur with the same time course as the action potential, it is theoretically possible to use optical techniques to measure membrane potentials. The previously described optical changes are relatively small and to measure a change in a single axon the results of many trials had to be averaged. We had already tried over a hundred fluorescent dyes, the best giving a signal-to-noise ratio of 2:1 during an action potential in a giant axon, when our attention was called to merocyanine dyes. One of these dyes gives a rather large signal, easily visible in a single sweep. This fluorescence change could provide a powerful tool for measuring membrane potential in systems where the use of electrodes is inconvenient or impossible.