ENHANCEMENT OF CYTOTOXICITY OF ACTIVE MACROPHAGES BY MYCOPLASMA - ROLE OF MYCOPLASMA-ASSOCIATED INDUCTION OF TUMOR NECROSIS FACTOR-ALPHA (TNF-ALPHA) IN MACROPHAGES
ENHANCEMENT OF CYTOTOXICITY OF ACTIVE MACROPHAGES BY MYCOPLASMA - ROLE OF MYCOPLASMA-ASSOCIATED INDUCTION OF TUMOR NECROSIS FACTOR-ALPHA (TNF-ALPHA) IN MACROPHAGES
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DOI：
10.1111/j.1348-0421.1990.tb01006.x
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发表时间：
1990-01-01
影响因子：
2.6
通讯作者：
MIYAZAKI, T
中科院分区：
文献类型：
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作者：
ARAI, S;FURUKAWA, M;MIYAZAKI, T
研究了几种支原体诱导肿瘤坏死因子-α(TNF-α)的活性，其中肺炎支原体是人类呼吸道传染病的病原体。纯化的BALB/c小鼠腹膜巨噬细胞与活的或不活的支原体共同培养时，对Meth A细胞的杀伤活性显著增强。巨噬细胞与肺炎支原体和莱氏肺泡球虫的培养上清液对肿瘤坏死因子α敏感的L细胞有较强的杀伤活性，而对肿瘤坏死因子不敏感的L细胞没有明显的杀伤活性。添加抗肿瘤坏死因子-α。抗血清完全抑制这些上清液的细胞毒活性，提示很大一部分细胞毒活性可能是由于肿瘤坏死因子-α所致。到目前为止，各种其他利用葡萄糖或精氨酸的支原体也显示出产生肿瘤坏死因子-α的强大活性。这些结果强烈提示支原体可能具有肿瘤坏死因子-α的活性。诱导可能与体内增强巨噬细胞的细胞毒活性和抵抗支原体感染有关。
Tumor necrosis factor-alpha (TNF-.alpha.)-inducing activity of several mycoplasmas including Mycoplasma pneumoniae, a causative agent in human respiratory infectious diseases, was investigated. Purified peritoneal macrophages from BALB/c mice markedly enhanced their cytotoxic activity to Meth A cells, when cultured with either viable or non-viable mycoplasmas. The supernatants of the macrophage culture with mycoplasmas, M. pneumoniae and Acholeplasma laidlawii, showed the potent cytotoxic activity to TNF-.alpha.-sensitive L cells but not to TNF-.alpha.-insensitive L cells. Addition of anti-TNF-.alpha. antiserum inhibited completely the cytotoxic activity of these supernatants, indicating that a major part of the cytotoxic activity might be due to TNF-.alpha.. Various other mycoplasmas, either glucose- or arginine-utilizing species, as far as tested showed also the potent activity to produce TNF-.alpha.. These results strongly suggest the possibility that mycoplasmas possess the activity of TNF-.alpha. induction which might be responsible for a part of enhancement of cytotoxic activity of macrophages and resistance to infection with mycoplasmas in vivo.