Electrokinetic trapping and concentration enrichment of DNA in a microfluidic channel
Electrokinetic trapping and concentration enrichment of DNA in a microfluidic channel
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DOI：
10.1021/ja0374776
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发表时间：
2003-10-29
影响因子：
15
通讯作者：
Crooks, RM
中科院分区：
文献类型：
--
作者：
Dai, JH;Ito, T;Crooks, RM
我们报告了一种简单而有效的方法，用于在微流控通道中丰富带电分析物的浓度。该方法依赖于对分析物的电动速度进行空间控制。具体地说，微流控系统的一个区域中缓冲溶液的电渗透(EO)速度与另一个区域中分析物的电泳(EP)速度相反。这导致EP将DNA输送到EP和EO速度相等和相反的位置。分析物的积累发生在这个位置。这种富集法在概念上有别于先前已报道的场放大堆积法、等速电泳法、胶束扫描法、尺寸排除法和其他方法。该方法不需要复杂的微结构，不需要对溶剂进行特殊操作，浓缩的分析物仍然留在溶液中，而不是被捕获在固体载体上。在尺寸为100μm×25μm×5 mm的流道中，20mer DNA的浓缩倍数可达800。达到这种程度的浓缩所需时间为300S，富集带的最小宽度为100μm。
We report a simple and efficient method for enriching the concentration of charged analytes within microfluidic channels. The method relies on exerting spatial control over the electrokinetic velocity of an analyte. Specifically, the electroosmotic (eo) velocity of the buffer solution in one region of the microfluidic system opposes the electrophoretic (ep) velocity of the analyte in the other region. This results in ep transport of DNA to the location where the ep and eo velocities are equal and opposite. Accumulation of the analyte occurs at this location. This enrichment method is conceptually distinct from field-amplification stacking, isotachophoresis, micelle sweeping, size exclusion, and other methods that have been previously reported. The method requires no complex microfabricated structures, no special manipulation of the solvent, and the enriched analyte remains in solution rather than being captured on a solid support. A concentration enrichment factor of 800 can be achieved for 20mer DNA in a fluidic channel having dimensions of 100 μm × 25 μm × 5 mm. The time required to achieve this level of enrichment is 300 s, and the enriched zone has a minimum width of 100 μm.