Multiple metal tolerance and biosorption of cadmium by Candida tropicalis isolated from industrial effluents: glutathione as detoxifying agent
Multiple metal tolerance and biosorption of cadmium by Candida tropicalis isolated from industrial effluents: glutathione as detoxifying agent
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DOI：
10.1007/s10661-010-1480-x
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发表时间：
2011-03
影响因子：
3
通讯作者：
A. Rehman;M. S. Anjum
中科院分区：
文献类型：
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作者：
A. Rehman;M. S. Anjum
研究了耐金属酵母热带假丝酵母从液体培养基和废水中吸收镉的能力。Cd ~（2+）对C. tropicalis为2，500 mg L− 1。该酵母还显示出对Zn 2+（1，400 mg L− 1）、Ni 2+（1，000 mg L− 1）、Hg 2+（1，400 mg L− 1）、Cu 2+（1，000 mg L− 1）、Cr 6+（1，200 mg L− 1）和Pb 2+（1，000 mg L− 1）的耐受性。酵母分离株表现出典型的生长曲线，但滞后期和对数期延长镉的存在下。酵母分离物在30°C和pH 8下显示出最佳生长。在含有100 mg L-1 Cd 2+的培养基中测定分离物的金属加工能力。在48、96和144 h后，热带假单胞菌对培养基中Cd ~（2+）的去除率分别为70%、85%和92%。Tropicalis对废水中Cd ~（2+）的去除率分别为40%和78%。在100 mg L− 1浓度下，镉使谷胱甘肽（GSH）和非蛋白巯基水平分别增加135%和134%。GSH合成的增加与金属耐受有关，GSH浓度的增加可能是C中高金属胁迫的标志。热带角对重金属离子具有抗性并能适应当地环境条件的tropicalis可用于金属解毒操作。
The ability of cadmium uptake by metal-resistant yeast,Candida tropicalis, from the liquid medium and wastewater was evaluated. The minimum inhibitory concentration of Cd2 +againstC. tropicaliswas 2,500 mg L− 1. The yeast also showed tolerance toward Zn2 +(1,400 mg L− 1), Ni2 +(1,000 mg L− 1), Hg2 +(1,400 mg L− 1), Cu2 +(1,000 mg L− 1), Cr6 +(1,200 mg L− 1), and Pb2 +(1,000 mg L− 1). The yeast isolate showed typical growth curves, but lag and log phases extended in the presence of cadmium. The yeast isolate showed optimum growth at 30°C and pH 8. The metal processing ability of the isolate was determined in a medium containing 100 mg L− 1of Cd2 +.C. tropicaliscould decline Cd2 +70%, 85%, and 92% from the medium after 48, 96, and 144 h, respectively.C. tropicaliswas also able to remove Cd2 +40% and 78% from the wastewater after 6 and 12 days, respectively. Cd produced an increase in glutathione (GSH) and nonprotein thiol levels by 135% and 134% at 100-mg L− 1concentration, respectively. An increase in the synthesis of GSH is involved in metal tolerance, and the presence of increasing GSH concentrations may be a marker for high metal stress inC. tropicalis.C. tropicalis, which is resistant to heavy metal ions and is adaptable to the local environmental conditions, may be employed for metal detoxification operations.