Metabolic features of rats resistant to a high-fat diet
Metabolic features of rats resistant to a high-fat diet
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DOI：
10.1016/j.orcp.2013.01.004
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发表时间：
2013-07-01
影响因子：
4.3
通讯作者：
Date, Y.
中科院分区：
文献类型：
--
作者：
Akieda-Asai, S.;Koda, S.;Date, Y.
目的：我们评估高脂饮食抵抗（DR）大鼠的代谢特征。方法：测量标准饲料喂养的对照大鼠、高脂饮食喂养的肥胖倾向（高脂饮食诱导的肥胖，DIO）和 DR 大鼠的体重、能量摄入、运动活动、耗氧量、血浆瘦素和脂质水平、内脏脂肪脂肪细胞的大小以及与脂质代谢相关的基因的 mRNA 水平。还进行了葡萄糖耐量和胰岛素耐量测试。结果：DIO 大鼠比 DR 和对照大鼠体重增加得更快；尽管能量摄入量相似，DR 大鼠的体重增加却比 DIO 大鼠少。三组之间的能量消耗没有差异。 DR 大鼠和对照大鼠的内脏脂肪细胞直径相似。与脂肪生成相关的基因（例如脂肪酸合酶和乙酰辅酶A羧化酶）的mRNA水平在DR组中往往低于对照组和DIO组大鼠，而参与脂肪酸β-氧化的肉碱棕榈酰转移酶1a的mRNA水平在DR组大鼠中高于其他组。 DIO大鼠表现出高胰岛素血症和葡萄糖耐受不良，而DR大鼠对胰岛素高度敏感。结论：DR大鼠表现出脂肪生成受到抑制，内脏脂肪中脂肪酸β-氧化加速。这些特征可能有助于 DR 大鼠的抗肥胖表型。 (C) 2013 年亚洲大洋洲肥胖研究协会。由爱思唯尔有限公司出版。保留所有权利。
Objective: We assessed the metabolic characteristics of high-fat-diet-resistant (DR) rats.Methods: Body weight, energy intake, locomotor activity, oxygen consumption, plasma leptin and lipid levels, size of visceral-fat adipocytes, and mRNA levels of genes related to lipid metabolism were measured in control rats fed standard chow and in obesity-prone (high-fat-diet-induced obesity, DIO) and DR rats fed a high-fat diet. Glucose tolerance and insulin tolerance tests were also performed.Results: DIO rats gained weight more rapidly than did DR and control rats; DR rats gained less weight than did DIO rats despite similar energy intake. Energy expenditure did not differ among the three groups. The diameter of visceral-fat adipocytes was similar in DR and control rats. mRNA levels of genes involved in lipogenesis, such as fatty acid synthase and acetyl-CoA carboxylase, tended to be lower in DR than in control and DIO rats, whereas those of carnitine palmitoyltransferase 1a, which is involved in fatty acid beta-oxidation, were greater in DR rats than in the other groups. DIO rats showed hyperinsulinemia and glucose intolerance, whereas DR rats had high sensitivity to insulin.Conclusion: DR rats show suppression of lipogenesis and acceleration of fatty acid beta-oxidation in the visceral fat. These characteristics likely contribute to the anti-obesity phenotype of DR rats. (C) 2013 Asian Oceanian Association for the Study of Obesity. Published by Elsevier Ltd. All rights reserved.