Nanoscale palladium metallization of DNA
Nanoscale palladium metallization of DNA
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DOI：
10.1002/(sici)1521-4095(200004)12:7
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发表时间：
2000-04
影响因子：
29.4
通讯作者：
J. Richter;R. Seidel;R. Kirsch;M. Mertig;W. Pompe;J. Plaschke;H. Schackert
中科院分区：
文献类型：
--
作者：
J. Richter;R. Seidel;R. Kirsch;M. Mertig;W. Pompe;J. Plaschke;H. Schackert
根据Massart [11]公布的程序在碱性溶液中制备磁铁矿胶体。向50 mL 1 M（CH 3）4 NOH× 5 H 2 O中加入含2.3 g（8.5 mmol）FeCl 3 × 6 H 2 O的4 mL H2 O和1.69 g（4.3 mmol）Fe（NH 4）2（SO 4）2的1 mL 2 M HCl水溶液。将所得黑色悬浮液在室温下搅拌1小时，然后在超声浴中超声处理1小时。然后将胶体以20 000 g离心1 h。倾析上清液，并通过超声处理将浆液重悬于20 mL水中，然后使其通过0.2 mm孔硝酸纤维素膜。按照O 'Regan等人[12]描述的程序，在氮气气氛下水解四异丙醇钛，制备二氧化钛溶胶。在氮气气氛下，将25 mL四异丙醇钛与4 mL异丙醇在滴液漏斗中混合。将该混合物在5分钟内缓慢加入到装有加热器、温度计和搅拌器的250 mL三颈烧瓶中的150 mL剧烈搅拌的双蒸馏去离子水中。在最后一次醇盐添加后10分钟，添加ImL的69%HNO 3。然后将该白色水解混合物在80 ℃下搅拌8小时以除去异丙醇，通过0.2 mm孔硝酸纤维素膜过滤，并超声处理1小时以产生具有蓝-白色着色的稳定胶体溶液。复合材料的制备和煅烧方法：通常，将切片的共聚物凝胶（约100 g）的样品在煅烧后的20分钟内进行煅烧。5 mm厚）加入到胶体溶胶中并放置所需的时间。取出负载胶体的凝胶，用水洗涤并使其在空气中干燥。使用NETZSCH STA 409 EP机器进行热重分析（TGA）测量。在空气中，在氧化铝坩埚中以5 K/min的速率将样品加热至800 C的最终温度。通过在Carbolite炉（ELF 11/6型）中以1 C/min ±1的加热速率加热至450或500 C的温度来煅烧矿化凝胶的大样品。分析方法：共聚物凝胶在水溶胀状态下的形态使用装备有低温台和能量分散X射线（EDX）的Jeol 6310 SEM检查。…
A magnetite colloid was prepared in alkaline solution according to the procedure published by Massart [11]. An aqueous solution containing 2.3 g (8.5 mmol) FeCl 3 ×6H 2 O in 4 mL H 2 O and 1.69 g (4.3 mmol) Fe(NH 4) 2 (SO 4) 2 in 1 mL of 2 M HCl, was added to 50 mL of 1 M (CH 3) 4 NOH×5H 2 O. The resulting black suspension was stirred for 1 h at room temperature and then sonicated in an ultrasonic bath for 1 h. The colloid was then centrifuged at 20 000 g for 1 h. The supernatant was decanted and the slurry resuspended in 20 mL water by sonication before being passed through a 0.2 mm pore cellulose nitrate membrane. A titanium dioxide sol was prepared by hydrolysis of titanium tetraiso-propoxide under a nitrogen atmosphere following the procedure described by O'Regan et al. [12]. 25 mL of titanium tetraisopropoxide was mixed with 4 mL of isopropanol in a dropping-funnel under a nitrogen atmosphere. This mixture was added slowly over a period of 5 min to 150 mL of vigorously stirred double-distilled, deionized water in a 250 mL three-neck flask equipped with heater, thermometer and stirrer. Ten minutes after the final alkoxide addition, 1 mL of 69 % HNO 3 was added. The white hydrolysis mixture was then stirred for 8 h at 80 C to remove the isopropanol, filtered through a 0.2 mm pore cellulose nitrate membrane, and sonicated for 1 h to produce a stable colloidal solution with a bluish-white coloration. Preparation of the Composites and Method of Calcination: Typically, a sample of the sliced copolymer gel (ca. 5 mm thick) was added to the colloidal sol and left for the desired period of time. The colloid-loaded gels were removed, washed with water and allowed to dry in air. Thermogravi-metric analysis (TGA) measurements were made using a NETZSCH STA 409EP machine. Samples were heated under air in an alumina crucible to a final temperature of 800 C at a rate of 5 K/min. Large samples of the miner-alized gels were calcined by heating to a temperature of 450 or 500 C in a Carbolite furnace (type ELF11/6) at a heating rate of 1 C min ±1. Analytical Methods: The morphology of the copolymer gel in the water-swollen state was examined using a Jeol 6310 SEM equipped with a cryo-stage and energy-dispersive X-ray (EDX). …