A novel luminol chemiluminescence induced by photoexcited ketones: A selective determination method for acetone in wastewater
A novel luminol chemiluminescence induced by photoexcited ketones: A selective determination method for acetone in wastewater
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DOI：
10.1016/j.talo.2021.100035
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发表时间：
2021-08
期刊：
影响因子：
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通讯作者：
Hitoshi Kodamatani;Daisuke Yoshimine;Takahiro Fujioka;R. Kanzaki;T. Tomiyasu
中科院分区：
文献类型：
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作者：
Hitoshi Kodamatani;Daisuke Yoshimine;Takahiro Fujioka;R. Kanzaki;T. Tomiyasu
最近有研究证实，光激发酮类如丙酮能促进发光氨及其类似物L-012的化学发光反应。在此基础上，建立了HPLC分离、在线紫外照射、鲁米诺CL检测的丙酮选择性测定方法。考察了不同的反应条件，包括鲁米诺试剂的种类、有机溶剂作为淋洗剂的使用以及反应的pH，以阐明CL反应的机理。结果表明，发光氨的CL反应是由光激发的丙酮产生丙酮酮基自由基引起的。通过在自来水厂水质保证中的应用，评价了丙酮测定新方法的性能。丙酮的检出限为0.03µM，定量限为0.08µM。在0.1 ~ 200µM的浓度范围内呈线性变化。添加5.0µM丙酮的废水和海水样品（n= 5）的回收率为87 ~ 108%。结果表明，该体系具有良好的灵敏度和选择性。
It has been recently confirmed that photoexcited ketones such as acetone promote the chemiluminescence (CL) reaction of luminol and its analog, L-012. Using this finding, a selective determination method for acetone was developed herein based on HPLC separation, online UV irradiation, and subsequent luminol CL detection. Various conditions, including the type of luminol reagent, the use of organic solvents as eluent modifiers, and the reaction pH, were investigated to elucidate the CL reaction mechanism. The results showed that the CL reaction of luminol is caused by an acetone ketyl radical produced from the photoexcited acetone. The performance of the novel acetone determination method was evaluated by its application in water quality assurance in water treatment plants. The method detection limit and the method quantification limit of acetone were 0.03 and 0.08 µM, respectively. A linear range was obtained in the concentration range of 0.1–200 µM. The range of recovery in the wastewater and seawater samples (n= 5) fortified with 5.0 µM acetone was 87–108%. These results demonstrate that the new luminol CL system is useful for its sensitivity and selectivity.