A genetic and biochemical approach to study trichothecene diversity in Fusarium sporotrichioides and Fusarium graminearum
A genetic and biochemical approach to study trichothecene diversity in Fusarium sporotrichioides and Fusarium graminearum
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DOI：
10.1006/fgbi.2001.1256
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发表时间：
2001-03-01
影响因子：
3
通讯作者：
Desjardins, AE
中科院分区：
文献类型：
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作者：
Brown, DW;McCormick, SP;Desjardins, AE
腐殖酸T-2毒素和脱氧雪腐镰刀菌烯醇（DON）是对动物和植物都有毒的天然真菌产物。它们在镰刀菌对作物植物的致病性中的重要性激发了人们努力理解导致二孢噻吩合成的遗传和生化机制。为了更好地了解拟孢毛镰孢T-2毒素的生物合成和F.禾谷镰刀菌中，我们比较了来自每种生物的23-kb核心拟南芥烯基因簇的核苷酸序列。这种比较遗传分析使我们能够预测由两个镰刀菌烯基因TRI 9和TRI 10编码的蛋白质，这些蛋白质以前从未在任何镰刀菌物种中描述过。基因结构的差异也与两个物种产生的多烯类型的差异有关。sporotrichioides TRI 7（FsTRI 7）是T-2毒素C-4位氧的乙酰化所必需的。禾谷镰刀菌TRI 7（FgTRI 7）是无功能的。这与FgTRI 7产物不是F中DON合成所需的事实一致。因为C-4不含氧。
The trichothecenes T-2 toxin and deoxynivalenol (DON) are natural fungal products that are toxic to both animals and plants. Their importance in the pathogenicity of Fusarium spp, on crop plants has inspired efforts to understand the genetic and biochemical mechanisms leading to trichothecene synthesis. In order to better understand T-2 toxin biosynthesis by Fusarium sporotrichioides and DON biosynthesis by F. graminearum, we compared the nucleotide sequence of the 23-kb core trichothecene gene cluster from each organism. This comparative genetic analysis allowed us to predict proteins encoded by two trichothecene genes, TRI9 and TRI10, that had not previously been described from either Fusarium species. Differences in gene structure also were correlated with differences in the types of trichothecenes that the two species produce, Gene disruption experiments showed that F. sporotrichioides TRI7 (FsTRI7) is required for acetylation of the oxygen on C-4 of T-2 toxin, Sequence analysis indicated that F. graminearum TRI7 (FgTRI7) is nonfunctional. This is consistent with the fact that the FgTRI7 product is not required for DON synthesis in F. graminearum because C-4 is not oxygenated.