Genome-wide screening by cDNA microarray of genes associated with matrix mineralization by human mesenchymal stem cells in vitro
Genome-wide screening by cDNA microarray of genes associated with matrix mineralization by human mesenchymal stem cells in vitro
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DOI：
10.1006/bbrc.2001.6196
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发表时间：
2002-01-11
影响因子：
3.1
通讯作者：
Nakamura, Y
中科院分区：
文献类型：
--
作者：
Doi, M;Nagano, A;Nakamura, Y
使用一个培养系统，促进骨髓间充质干细胞的成骨分化，我们分析了基因表达谱在矿化过程中的cDNA微阵列系统由23,040个基因组成。我们比较了在含有0.1 μ M地塞米松、0.05 mM抗坏血酸-2-磷酸和10 mM β-甘油磷酸的组合、仅地塞米松、抗坏血酸-2-磷酸加（β）甘油磷酸的培养基或不含任何这些成骨补充剂的培养基中孵育第3、15和27天的细胞表达谱。组织化学分析显示，在所有三种试剂的存在下孵育的细胞的成骨分化，但在其他培养物中没有。表达谱的比较揭示了55个基因的转录刺激和82个基因的抑制中超过20，000检查。一组差异表达的基因，我们在这里报告应有助于更好地了解周围的基质间充质干细胞的矿化过程。(C)2002年，Elsevier Science。
Using a culture system that facilitates osteogenic differentiation of bone marrow-derived human mesenchymal stem cells, we analyzed gene-expression profiles during the mineralization process by means of a cDNA microarray system consisting of 23,040 genes. We compared expression profiles of the cells at days 3, 15, and 27 of incubation in media containing either a combination of 0.1 muM dexamethasone, 0.05 mM ascorbic acid-2-phosphate, and 10 mM beta-glycerophosphate, dexamethasone only, ascorbic acid-2-phosphate plus (-beta)glycerophosphate, or medium without any of these osteogenic supplements. Histochemical analysis revealed osteogenic differentiation of cells incubate in the presence of all three agents, but not in the other cultures. Comparison of the expression profiles disclosed transcriptional stimulation of 55 genes and repression of 82 genes among more than 20,000 examined. A set of differentially expressed genes we report here should contribute to a better understanding of the process of mineralization in the matrix surrounding human mesenchymal stem cells. (C) 2002 Elsevier Science.