miR-19b-3p integrates Jak-Stat signaling pathway through Plzf to regulate self-renewal in dairy goat male germline stem cells
miR-19b-3p integrates Jak-Stat signaling pathway through Plzf to regulate self-renewal in dairy goat male germline stem cells
复制标题
miR-19b-3p通过Plzf整合Jak-Stat信号通路调节奶山羊雄性种系干细胞的自我更新
DOI：
10.1016/j.biocel.2018.10.010
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发表时间：
2018-12-01
影响因子：
4
通讯作者：
Hua, Jinlian
中科院分区：
文献类型：
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作者：
Clotaire, Daguia Zambe John;Du, Xiaomin;Hua, Jinlian
Jak-Stat途径是第一个被发现刺激精原干细胞（SSCs）自我更新和维持活性的途径。最近的研究表明，stat 3是这一通路中的关键基因，它可以调控雄性生殖干细胞的自我更新。在我们之前的研究中，我们证明了miR-19 b-3 p通过Plzf诱导细胞增殖并减少异染色质，Plzf还调节细胞自我更新和分化之间的平衡。由于miRNA可以靶向多个基因，为了更好地了解SSCs的关键转录因子Plzf，我们进行了微阵列研究，发现miR-19 b-3 p通过Plzf整合Jak-Stat来调节细胞自我更新。我们的研究结果表明，当Plzf下调时，miR-19 b-3 p诱导Jak-Stat; Plzf的过表达逆转了这一趋势，并显示Plzf和GHR之间存在反馈（-/ +）。与其各自的对照相比，在miR-19 b3 p和Plzf过表达的两个组中分析的细胞自我更新标志物CD 49 f、GFR α 1、Oct 4和cKIT证实了miR-19 b-3 p通过Plzf调节山羊雄性生殖系干细胞中的细胞多能性和自我更新。总之，我们的发现揭示了miR-19 b-3 p通过Plzf控制Jak-Stat信号传导。
Jak-Stat pathway is the first pathway identified to stimulate spermatogonial stem cells (SSCs) self-renewal and maintenance activity. Recent studies have showed that stat3 a crucial gene implicated in this pathway can regulate self-renewal in male germline stem cell. In our previous study, we demonstrated that miR-19b-3p induces cell proliferation and reduces heterochromatin through Plzf which also regulates the balance between cell self-renewal and differentiation. Because miRNA can target several genes and to understand more about Plzf, a crucial transcription factor of SSCs, we performed microarray and found that miR-19b-3p integrate Jak-Stat through Plzf to regulate cell self-renewal. Our results demonstrated that miR-19b-3p induces Jak-Stat when Plzf is downregulated; overexpression of Plzf reversed the trend and shown an existence of feedback (-/ + ) between Plzf and GHR. The cell self-renewal markers CD49f, GFR alpha 1, Oct4 and cKIT analyzed in the both groups miR-19b3p and Plzf-overexpressing compared to their respective control confirm miR-19b-3p regulates cell pluripotency and self-renewal in goat male germline stem cells through Plzf. Together our finding revels that miR-19b-3p control Jak-Stat signaling through Plzf.