Global transcript analysis of rice leaf and seed using SAGE technology
Global transcript analysis of rice leaf and seed using SAGE technology
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DOI：
10.1046/j.1467-7652.2003.00026.x
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发表时间：
2003-07-01
影响因子：
13.8
通讯作者：
Dunwell, JM
中科院分区：
文献类型：
--
作者：
Gibbings, JG;Cook, BP;Dunwell, JM
我们从水稻的成熟叶片和未成熟种子组织中收集了两组基因表达谱。利用基因表达序列分析（SAGE）技术对粳稻品种Nipponbare进行分析。分析共发现50 519个SAGE标签，对应15 131个唯一转录本。其中，绝大多数（接近70%）只在两个库中出现一次。出乎意料的是，在叶片文库中最丰富的转录本（约占总数的3%）来自3型金属硫蛋白基因。两种组织中丰富的标签物种的总体频率谱差异很大，表明种子组织表现出非典型的基因表达模式，其特征是编码储存蛋白的少量转录本过丰富。高比例（接近80%）的丰度标签（大于或等于9）与参考稻米EST数据库中的条目匹配，而低丰度标签的匹配则少得多。对两种组织共有的单例转录本进行了整理，以总结在水稻组织中组成表达的低丰度转录本。最后也是最令人惊讶的是，大量的标签被发现编码反义。转录本，这一发现表明了一种新的基因调控机制，并可能对在转基因技术中使用反义结构体产生影响。
We have compiled two comprehensive gene expression profiles from mature leaf and immature seed tissue of rice (Oryza sativa ssp. japonica cultivar Nipponbare) using Serial Analysis of Gene Expression (SAGE) technology. Analysis revealed a total of 50 519 SAGE tags, corresponding to 15 131 unique transcripts. Of these, the large majority (similar to70%) occur only once in both libraries. Unexpectedly, the most abundant transcript (similar to3% of the total) in the leaf library was derived from a type 3 metallothionein gene. The overall frequency profiles of the abundant tag species from both tissues differ greatly and reveal seed tissue as exhibiting a non-typical pattern of gene expression characterized by an over abundance of a small number of transcripts coding for storage proteins. A high proportion (similar to80%) of the abundant tags (greater than or equal to9) matched entries in our reference rice EST database, with many fewer matches for low abundant tags. Singleton transcripts that are common to both tissues were collated to generate a summary of low abundant transcripts that are expressed constitutively in rice tissues. Finally and most surprisingly, a significant number of tags were found to code for antisense. transcripts, a finding that suggests a novel mechanism of gene regulation, and may have implications for the use of antisense constructs in transgenic technology.