Apoptosis-associated markers and clinical outcome in human oral squamous cell carcinomas
Apoptosis-associated markers and clinical outcome in human oral squamous cell carcinomas
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DOI：
10.1111/j.1600-0714.2008.00642.x
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发表时间：
2008-07-01
影响因子：
3.3
通讯作者：
Yamamoto, Etsuhide
中科院分区：
文献类型：
--
作者：
Kato, Koroku;Kawashiri, Shuichi;Yamamoto, Etsuhide
背景：细胞凋亡是一种基因调控的细胞死亡，在正常或恶性组织中参与细胞的缺失。Bcl-2家族的蛋白质在细胞凋亡的控制中起关键作用，并执行促细胞凋亡和抗细胞凋亡功能。本研究旨在探讨Bcl-2和Bax在口腔鳞状细胞癌（OSCC）浸润前沿的表达及其临床病理意义。方法：采用免疫组化方法检测五十六例OSCC浸润前沿石蜡包埋标本中Bcl-2和Bax的表达。收集临床病理数据并分析患者生存情况。结果：未发现Bcl-2或Bax表达与临床变量之间存在显着关系。Bcl-2阳性表达者预后较阴性表达者差，但差异无统计学意义。Bax表达阳性者预后明显好于Bax阴性者（P < 0.05）。单因素分析显示T分期、肿瘤浸润方式与Bax表达有显著相关性。多因素分析显示，肿瘤侵袭方式和Bax表达是显着的独立变量。Bax表达被认为是最强的独立预后参数。Bcl-2表达阴性而Bax表达阳性者预后较好（P < 0.005）。结论：Bax在口腔鳞癌浸润前沿的表达是判断预后的重要指标，因此研究Bcl-2和Bax在口腔鳞癌中的表达具有重要意义。
Background: Apoptosis is a genetically regulated cell death involved in the deletion of cells in normal or malignant tissues. Proteins of the Bcl-2 family play a key role in the control of apoptosis and carry out both pro-apoptotic and anti-apoptotic functions. The present study evaluated the prognostic value of Bcl-2 and Bax expression at the invasive front of oral squamous cell carcinoma (OSCC), taking clinicopathological findings into account.Methods: Fifty-six specimens of OSCC were randomly selected, and Bcl-2 and Bax expression was evaluated by immunohistochemistry in formalin-fixed, paraffin-embedded pre-treated specimens at the invasive front of OSCC. Clinicopathological data were gathered and patient survival was analysed.Results: No significant relationship was found between Bcl-2 or Bax expression and clinical variables. Patients with Bcl-2 expression had a worse prognosis than those without Bcl-2 expression, but the difference did not reach statistical significance. Patients with Bax expression had a significantly better prognosis than those without Bax expression (P < 0.05). In univariate analyses, T category, mode of cancer invasion and Bax expression showed significant correlations. Multivariate analysis revealed that the mode of cancer invasion and Bax expression were significant and independent variables. Bax expression was found to be the strongest independent prognostic parameter. Patients with negative Bcl-2 expression and positive Bax expression had a significantly better prognosis (P < 0.005).Conclusions: We suggest that Bax expression at the invasive front of OSCC is a significant predictor of prognosis and that it is therefore important to investigate the expression of Bcl-2 and Bax in this disease.