Multimodal nonlinear optical microscopy with shaped 10 fs pulses.
Multimodal nonlinear optical microscopy with shaped 10 fs pulses.
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DOI：
10.1364/oe.22.028790
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发表时间：
2014-11
期刊：
影响因子：
3.8
通讯作者：
J. Rehbinder;L. Brückner;Alexander Wipfler;T. Buckup;M. Motzkus
中科院分区：
文献类型：
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作者：
J. Rehbinder;L. Brückner;Alexander Wipfler;T. Buckup;M. Motzkus
我们证明了使用形状的10 fs脉冲多模显微镜。宽带振荡器和脉冲整形器的组合提供了一种灵活的光源，该光源可以针对样品中产生的各种非线性效应进行优化，无论是针对信号强度还是针对选择性。虽然最高的非线性产生效率是通过最短的脉冲实现的，但更复杂的波形可以解决样品中的特定过渡，以获得更好的对比度。这表明实验与成像的苔藓叶和人体皮肤活检，使用相干反斯托克斯拉曼散射，双光子荧光和二次谐波产生信号。
We demonstrate the use of shaped 10 fs pulses for multimodal microscopy. The combination of a broadband oscillator and a pulse shaper provides a flexible light source that can be optimized for various nonlinear effects produced in the sample, either for signal intensity or for selectivity. While the highest nonlinear generation efficiency is achieved with the shortest pulses, more complex waveforms address specific transitions in the sample for better contrast. This is shown experimentally with the imaging of a moss leaf and of human skin biopsies using coherent anti-Stokes Raman scattering, two-photon fluorescence and second harmonic generation signals.