Identification and characterization of rice blast resistance gene Pid4 by a combination of transcriptomic profiling and genome analysis
Identification and characterization of rice blast resistance gene Pid4 by a combination of transcriptomic profiling and genome analysis
复制标题
结合转录组分析和基因组分析对稻瘟病抗性基因 Pid4 进行鉴定和表征（在线）
DOI：
10.1016/j.jgg.2018.10.007
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发表时间：
2018-12-20
影响因子：
5.9
通讯作者：
Chen, Xuewei
中科院分区：
文献类型：
--
作者：
Chen, Zhixiong;Zhao, Wen;Chen, Xuewei
基于图谱的植物抗病(R)基因克隆是耗时的。在这里，我们报道了利用比较转录图谱和全基因组序列分析分离的BLAST R基因Pid4。PID4编码一个富含亮氨酸的螺旋状核苷酸结合位点重复序列(CC-NBS-LRR)蛋白，在水稻品种地古中的不同发育阶段都有结构性表达。Pid4蛋白定位于胞核和胞浆。将Pid4基因导入感病水稻品种，可获得对叶瘟和穗瘟病的小种特异性抗性。氨基酸序列比较和抗稻瘟病光谱测试表明，Pid4是一个新的R基因，不同于以往报道的位于同一基因簇中的R基因。为了便于在不同水稻品种中鉴定Pid4，开发了Pid4 Indel标记。我们证明了利用转录图谱结合全基因组序列分析可以快速分离植物R基因。版权所有(C)2018，中国科学院遗传与发育生物学研究所，中国遗传学会。爱思唯尔有限公司和科学出版社出版。版权所有。
Map-based cloning of plant disease resistance (R) genes is time-consuming. Here, we reported the isolation of blast R gene Pid4 using comparative transcriptomic profiling and genome-wide sequence analysis. Pid4 encodes a coiled-coil nucleotide-binding site leucine-rich repeat (CC-NBS-LRR) protein and is constitutively expressed at diverse developmental stages in the rice variety Digu. The Pid4 protein is localized in both the nucleus and cytoplasm. Introduction of Pid4 into susceptible rice cultivars confers race-specific resistance to leaf and neck blast. Amino acid sequence comparison and blast resistance spectrum tests showed that Pid4 is a novel R gene, different from the previously reported R genes located in the same gene cluster. A Pid4 Indel marker was developed to facilitate the identification of Pid4 in different rice varieties. We demonstrated that a plant R gene can be quickly isolated using transcriptomic profiling coupled with genome-wide sequence analysis. Copyright (C) 2018, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and Genetics Society of China. Published by Elsevier Limited and Science Press. All rights reserved.